PBX1 antibody (Internal Region)

Catalog No. ABIN498074

Product Details anti-PBX1 Antibody

Target: PBX1 (Pre-B-Cell Leukemia Homeobox Protein 1 (PBX1))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This PBX1 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Sequence: C-ATNVSAHGSQ ANSPS

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Canine (Dog).
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide from the internal region of the protein sequence according to NP_002576.1. Genename: PBX1

Application Details

Application Notes: Peptide ELISA: Detection Limit: 1/32000. Western blot: 0.5-1.5 μg/mL. Detects a band of Approx 45 kDa in lysates of cell line K562.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Species Reactivity: Tested: Human. Expected from sequence similarity: Mouse, Rat, Canine (Dog). Add. Information: Calculated Molecular Weight: 46.5 kDa according to NP_002576.1

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for PBX1

Target: PBX1 (Pre-B-Cell Leukemia Homeobox Protein 1 (PBX1))

Alternative Name: PBX1 (PBX1 Products)

Synonyms: fi29e01 antibody, pbx1b antibody, wu:fi29e01 antibody, zgc:55265 antibody, 2310056B04Rik antibody, AI848790 antibody, AU015730 antibody, D230003C07Rik antibody, Pbx-1 antibody, Pbx1a antibody, Pbx1b antibody, PBX1A antibody, Xpbx1 antibody, Xpbx1b antibody, PBX homeobox 1 antibody, pre-B-cell leukemia homeobox 1a antibody, pre B cell leukemia homeobox 1 antibody, pre-B-cell leukemia homeobox 1 S homeolog antibody, PBX1 antibody, pbx1a antibody, Pbx1 antibody, pbx1.S antibody

Background: This nonactivating protein which could be a repressor, binds the sequence 5'-ATCAATCAA-3'. It is converted into a potent transcriptional activator by the (1,19) translocation. PBX1 May have a role in steroidogenesis and subsequently sexual development and differentiation, and is expressed in all tissues except in cells of the B and T lineage. PBX1 is involved in a form of pre-B-cell acute lymphoblastic leukemia (B-ALL) (25 % of human pediatric cases) through a chromosomal translocation t(1,19)(q23,p13.3) which involves PBX1 and E2A genes. E2A-PBX1 transforms cells by constitutively activating transcription of genes regulated by PBX1 or by other members of the PBX protein family. PBX1 belongs to the TALE/PBX homeobox family, and contains 1 homeobox domain.Synonyms: Homeobox protein PBX1, Homeobox protein PRL, Pre-B-cell leukemia transcription factor 1

Gene ID: 5087

NCBI Accession: NP_002576

UniProt: P40424