ETFDH antibody (Internal Region)

Catalog No. ABIN498086

Product Details anti-ETFDH Antibody

Target: ETFDH (Electron-Transferring-Flavoprotein Dehydrogenase (ETFDH))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat, Dog, Cow, Rabbit

Host: Goat

Clonality: Polyclonal

Conjugate: This ETFDH antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Canine (Dog), Bovine and Rabbit.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence C-EHDQPAHLTLRD, from the internal region of the protein sequence according to NP_004444.2. Genename: ETFDH

Application Details

Application Notes: Peptide ELISA: Detection Limit: 1/2000. Western blot: 1-3 μg/mL. Detects a band of Approx 70 kDa in Human, Mouse and Rat Heart lysates.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. Species Reactivity: Tested: Human. Expected from sequence similarity: Mouse, Rat, Canine (Dog), Bovine and Rabbit. Add. Information: Calculated Molecular Weight: 68.0 kDa according to NP_004444.2

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for ETFDH

Target: ETFDH (Electron-Transferring-Flavoprotein Dehydrogenase (ETFDH))

Alternative Name: ETFDH (ETFDH Products)

Synonyms: ETFQO antibody, MADD antibody, 0610010I20Rik antibody, AV001013 antibody, MGC81928 antibody, zgc:92093 antibody, wu:fa05a11 antibody, wu:fb71g06 antibody, MGC69479 antibody, DDBDRAFT_0206234 antibody, DDBDRAFT_0267019 antibody, DDB_0206234 antibody, DDB_0267019 antibody, ETF-QO antibody, electron transfer flavoprotein dehydrogenase antibody, electron transferring flavoprotein, dehydrogenase antibody, electron transfer flavoprotein dehydrogenase L homeolog antibody, electron-transferring-flavoprotein dehydrogenase antibody, electron transfer flavoprotein-ubiquinone oxidoreductase antibody, Electron-transferring-flavoprotein dehydrogenase antibody, ETFDH antibody, Etfdh antibody, etfdh.L antibody, etfdh antibody, CBU_1120 antibody, ZMO1184 antibody, RPE_4421 antibody, Daci_4310 antibody, Bphyt_2765 antibody, Dtpsy_1751 antibody, Za10_0145 antibody, Mesop_5359 antibody

Background: ETFDH or Electron-transferring-flavoprotein dehydrogenase in the inner mitochondrial membrane accepts electrons from electron-transfer flavoprotein which is located in the mitochondrial matrix and reduces ubiquinone in the mitochondrial membrane. The protein is synthesized as a 67- kDa precursor which is targeted to mitochondria and processed in a single step to a 64- kDa mature form located in the mitochondrial membrane. Deficiency in electron-transferring-flavoprotein dehydrogenase have been demonstrated in some patients with type II glutaricacidemia. ETFDH accepts electrons from ETF and reduces ubiquinone.Synonyms: ETF-QO, ETF-ubiquinone oxidoreductase, Electron transfer flavoprotein-ubiquinone oxidoreductase

Gene ID: 2110

NCBI Accession: NP_004444

UniProt: Q16134

Pathways: Monocarboxylic Acid Catabolic Process