DDX3X antibody

Catalog No. ABIN498119

Product Details anti-DDX3X Antibody

Target: DDX3X (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 3, X-Linked (DDX3X))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DDX3X antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody detects endogenous levels of DDX3 protein.

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity chromatography using epitope-specific immunogen

Purity: > 95 % (by SDS-PAGE)

Application Details

Application Notes: Western blot: 1: 500 - 1: 1000. Immunohistochemistry on paraffin sections: 1: 50 - 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for DDX3X

Target: DDX3X (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 3, X-Linked (DDX3X))

Alternative Name: DDX3X (DDX3X Products)

Synonyms: DBX antibody, DDX14 antibody, DDX3 antibody, HLP2 antibody, D1Pas1-rs2 antibody, Ddx3 antibody, Fin14 antibody, An3 antibody, pl10 antibody, DDX3X antibody, DEAD-box helicase 3, X-linked antibody, DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 3, X-linked antibody, DEAD-box helicase 3, X-linked L homeolog antibody, DDX3X antibody, Ddx3x antibody, ddx3x.L antibody, ddx3x antibody

Background: DDX3 contains all of the motifs of the DEAD box family of RNA helicases, including the Asp/Glu/Ala/Asp sequence that gives the protein family its name and distinguishes it from other RNA helicases. DDX3 is localized to the X chromosome and has a highly conserved functional homolog (DBY) on the Y chromosome. DDX3 is thought to be involved in RNA splicing, RNA transport, and translation initiation. It is also involved in cell growth control and is deregulated in hepatitis virus associated hepatocellular carcinoma. Recent experiments suppressing DDX3 expression blocked HIV1 RNA export from the nucleus, suggesting that DDX3 functions as a shuttling protein that transports the HIV1 protein Rev and its cofactor CRM1 from the nucleus to the cytoplasm.Synonyms: ATP-dependent RNA helicase DDX3X, DBX, DDX3, DEAD box X isoform, DEAD box protein 3 X-chromosomal, DEAD3, Embryonic RNA helicase, HLP2, Helicase-like protein 2

Molecular Weight: approx. 75 kDa

Gene ID: 1654

NCBI Accession: NP_001347

UniProt: O00571

Pathways: Ribonucleoprotein Complex Subunit Organization, Positive Regulation of Endopeptidase Activity, Negative Regulation of intrinsic apoptotic Signaling, Ribosome Assembly