JAK3 antibody
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- Target See all JAK3 Antibodies
- JAK3 (Janus Kinase 3 (JAK3))
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Reactivity
- Human, Mouse, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This JAK3 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Specificity
- This antibody detects endogenous levels of JAK3 protein.
- Cross-Reactivity (Details)
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Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human. - Purification
- Affinity chromatography using epitope-specific immunogen
- Purity
- > 95 % (by SDS-PAGE)
- Top Product
- Discover our top product JAK3 Primary Antibody
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- Application Notes
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Western blot: 1: 500 - 1: 1000. Immunohistochemistry on paraffin sections: 1: 50 - 1: 200. Immunoflourescence: 1: 50 - 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 1.0 mg/mL
- Buffer
- Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.2
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.
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- Target
- JAK3 (Janus Kinase 3 (JAK3))
- Alternative Name
- Tyrosine-Protein Kinase JAK3 (JAK3 Products)
- Synonyms
- JAK-3 antibody, JAK3_HUMAN antibody, JAKL antibody, L-JAK antibody, LJAK antibody, fae antibody, wil antibody, RATJAK3 antibody, JAK antibody, Janus kinase 3 antibody, JAK3 antibody, Jak3 antibody
- Background
- Jak3 (Janus Kinase 3) belongs to the family of non-receptor Janus tyrosine kinases, which regulate a spectrum of cellular functions downstream of activated cytokine receptors in the lympho-hematopoietic system. Immunological stimuli, such as interferons and cytokines, induce recruitment of Stat transcription factors to cytokine receptor-associated JAK3. JAK3 then phosphorylates proximal Stat factors, which subsequently dimerize, translocate to the nucleus and bind to cis elements upstream of target gene promoters to regulate transcription. The canonical JAK/Stat pathway is integral to maintaining a normal immune system, stimulating proliferation, differentiation, survival and host resistance to pathogens. Altering JAK/Stat signaling to reduce cytokine induced pro-inflammatory responses represents an attractive target for anti-inflammatory therapies.Synonyms: JAK-3, Janus kinase 3, L-JAK, Leukocyte janus kinase
- Molecular Weight
- approx. 125 kDa
- Gene ID
- 3718
- NCBI Accession
- NP_000206
- UniProt
- P52333
- Pathways
- JAK-STAT Signaling, RTK Signaling, Response to Growth Hormone Stimulus, Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response, Protein targeting to Nucleus, Activated T Cell Proliferation, Unfolded Protein Response
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