CSTA antibody

Catalog No. ABIN498170

Product Details anti-CSTA Antibody

Target: CSTA (Cystatin A (Stefin A) (CSTA))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CSTA antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody detects endogenous levels of Cystatin A protein.

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Affinity chromatography using epitope-specific immunogen

Purity: > 95 % (by SDS-PAGE)

Application Details

Application Notes: Western blot: 1: 500 - 1: 1000. Immunohistochemistry on paraffin sections: 1: 50 - 1: 200. Immunoflourescence: 1: 50 - 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for CSTA

Target: CSTA (Cystatin A (Stefin A) (CSTA))

Alternative Name: Cystatin-A (CSTA Products)

Synonyms: AREI antibody, STF1 antibody, STFA antibody, CST antibody, CSTA antibody, ATCYSA antibody, FL3-27 antibody, T20B5.8 antibody, T20B5_8 antibody, cystatin A antibody, cystatin A antibody, cystatin A1 antibody, cystatin-A antibody, CSTA antibody, Csta1 antibody, Csta antibody, CYSA antibody, LOC100725920 antibody

Background: Cystatin A (also designated STF1, STFA, stefin A or cystatin AS) and cystatin B (also designated PME, CST6, STFB, CPI-B, stefin B and liver thiol proteinase inhibitor) are thiol protease inhibitors that form complexes with papain and the cathepsins B, H and L. Cystatin A, a cytoplasmic protein, is one of the precursor proteins of the cornified cell envelope in keratinocytes and plays a role in epidermal development and maintenance. Cystatin B protects against intracellular proteases leaking out of lysosomes and is primarily expressed in heart, liver and kidney.Synonyms: CSTA, Cystatin-AS, STF1, STFA, Stefin-A

Molecular Weight: approx. 11 kDa

Gene ID: 1475

NCBI Accession: NP_005204

UniProt: P01040

Pathways: Response to Water Deprivation