Phospholipase C gamma 1 antibody

Catalog No. ABIN498180

Product Details anti-Phospholipase C gamma 1 Antibody

Target: Phospholipase C gamma 1 (PLCG1) (phospholipase C, gamma 1 (PLCG1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Phospholipase C gamma 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody detects endogenous levels of PLC γ1 protein.

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity chromatography using epitope-specific immunogen

Purity: > 95 % (by SDS-PAGE)

Application Details

Application Notes: Western blot: 1: 500 - 1: 1000. Immunohistochemistry on paraffin sections: 1: 50 - 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS) with 0.05 % sodium azide, approx. pH 7.2

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for Phospholipase C gamma 1

Target: Phospholipase C gamma 1 (PLCG1) (phospholipase C, gamma 1 (PLCG1))

Alternative Name: PLCG1 (PLCG1 Products)

Synonyms: NCKAP3 antibody, PLC-II antibody, PLC1 antibody, PLC148 antibody, PLCgamma1 antibody, plc1 antibody, nckap3 antibody, plc-ii antibody, plc148 antibody, plcgamma1 antibody, PPLCA antibody, AI894140 antibody, Cded antibody, Plc-1 antibody, Plc-gamma1 antibody, Plcg-1 antibody, fj46f04 antibody, wu:fj46f04 antibody, wu:fj57a05 antibody, phospholipase C gamma 1 antibody, phospholipase C gamma 1 L homeolog antibody, 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase gamma-1 antibody, phospholipase C, gamma 1 antibody, PLCG1 antibody, plcg1.L antibody, LOC100633163 antibody, Plcg1 antibody, plcg1 antibody

Background: Phosphoinositide-specific phospholipase C (PLC) plays a crucial role in the initiation of receptor mediated signal transduction through the generation of the two second messengers, inositol 1,4,5-triphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. There are many mammalian PLC isozymes, including PLC β1, PLC β2, PLC β3, PLC β4, PLC γ1, PLC γ2, PLC δ1, PLC δ2 and PLC ε. PLC γ1 is widely distributed in bronchiolar epithelium, type I and II pneumocytes and fibroblasts of the interstitial tissue. Actinregulatory protein Villin is tyrosine phosphorylated and associates with PLC γ1 in the brush border of intestinal epithelial cells. Villin regulates PLC γ1 activity by modifying its own ability to bind phosphatidylinositol 4,5-biphosphate. PLC γ1 binds α1β1 Integrin and modulates α1β1 Integrin-specific adhesion. PLC γ1 and Ca2+ play a direct role in VEGF-regulated endothelial growth, however this signaling pathway is not linked to FGF-mediated effects in primary endothelial cells.Synonyms: 1-phosphatidylinositol-4, 5-bisphosphate phosphodiesterase gamma-1, PLC gamma1 Phospholipase C-II, PLC-148, PLC-II, PLC-gamma-1, PLC1, Phosphoinositide phospholipase C-gamma-1, Phospholipase C-gamma-1

Molecular Weight: approx. 155 kDa

Gene ID: 5335

NCBI Accession: NP_877963

UniProt: P19174

Pathways: RTK Signaling, WNT Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, Inositol Metabolic Process, Myometrial Relaxation and Contraction, Regulation of Muscle Cell Differentiation, Regulation of G-Protein Coupled Receptor Protein Signaling, Skeletal Muscle Fiber Development, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, VEGFR1 Specific Signals, VEGF Signaling