DAPP1 antibody

Catalog No. ABIN498231

Product Details anti-DAPP1 Antibody

Target: DAPP1 (Dual Adaptor of Phosphotyrosine and 3-phosphoinositides (DAPP1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DAPP1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody detects endogenous levels of DAPP1 protein.

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse.

Purification: Affinity Chromatography using epitope-specific immunogen.

Application Details

Application Notes: Western blot: 1: 500 - 1: 1000. Immunohistochemistry on paraffin sections: 1: 50approx. 1: 200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for DAPP1

Target: DAPP1 (Dual Adaptor of Phosphotyrosine and 3-phosphoinositides (DAPP1))

Alternative Name: DAPP1 (DAPP1 Products)

Synonyms: BAM32 antibody, DAPP1 antibody, zgc:111998 antibody, bam32 antibody, Bam32 antibody, dual adaptor of phosphotyrosine and 3-phosphoinositides 1 antibody, dual adaptor of phosphotyrosine and 3-phosphoinositides antibody, dual adaptor for phosphotyrosine and 3-phosphoinositides 1 antibody, DAPP1 antibody, Dapp1 antibody, dapp1 antibody

Background: B cell adapter molecule (BAM32) is also designated a dual adapter for phosphotyrosine and 3-phosphotyrosine, and 3-phosphoinositide (DAPP1) or B lymphocyte adapter protein. BAM32 is a B cell-associated adapter that is crucial for B cell antigen receptor signaling regulation. BAM32 interacts with Ptdlns and PLC γ2 and, upon B cell activation, the protein is phosphorylated on tyrosine residues. It is a mainly cytoplasmic protein that can translocate to the cell membrane after cell stimulation. BAM32, which contains one PH domain and one SH2 domain, is primarily expressed in placenta and lung tissues, but can also be detected in heart, liver, pancreas and brain.Synonyms: BAM32, DKFZp667E0716, Dual adapter for phosphotyrosine and 3-phosphotyrosine and 3-phosphoinositide, HSPC066, hDAPP1

Molecular Weight: approx. 32 kDa

Gene ID: 27071

NCBI Accession: NP_055210

UniProt: Q9UN19

Pathways: BCR Signaling