Cyclin D1 antibody

Catalog No. ABIN498398

Product Details anti-Cyclin D1 Antibody

Target: Cyclin D1 (CCND1)

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cyclin D1 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: This antibody detects endogenous levels of Cyclin D1 protein (region surrounding Asp86)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Purity: > 95 % by SDS-PAGE

Application Details

Application Notes: Western Blot: 1/500 - 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

References for anti-Cyclin D1 antibody (ABIN498398)

Goyari, Devi, Bengyella, Khan, Sharma, Kalita, Talukdar: "Unveiling the optimal parameters for cellulolytic characteristics of Talaromyces verruculosus SGMNPf3 and its secretory enzymes." in: Journal of applied microbiology, Vol. 119, Issue 1, pp. 88-98, (2016) (PubMed).

Smith, Bhattacharya, Williams, Dixon, Powell, Erkina, Erkine: "High-throughput screening system for inhibitors of human Heat Shock Factor 2." in: Cell stress & chaperones, Vol. 20, Issue 5, pp. 833-41, (2016) (PubMed).

Kushima, Sekine, Matsubara, Taniguchi, Ikegami, Tsuda: "Gastric adenocarcinoma of the fundic gland type shares common genetic and phenotypic features with pyloric gland adenoma." in: Pathology international, Vol. 63, Issue 6, pp. 318-25, (2015) (PubMed).

Brackeva, De Punt, Kramer, Costa, Verhaeghen, Stangé, Sadones, Xavier, Aerts, Gorus, Martens: "Potential of UCHL1 as biomarker for destruction of pancreatic beta cells." in: Journal of proteomics, Vol. 117, pp. 156-67, (2015) (PubMed).

Huang, Huang, Li, He, Chen, Yu, Chen, Zhang, Shi, Shan: "Correlation between tuberous sclerosis complex 2 and glycogen synthase kinase 3 beta levels, and outcomes of patients with hepatocellular carcinoma treated by hepatectomy." in: Hepatology research : the official journal of the Japan Society of Hepatology, Vol. 44, Issue 11, pp. 1142-50, (2014) (PubMed).

Zhang, Fong, Yu, Chen, Wei, Koon, Lau, Leung, Lau, Fung, Yang et al.: "Herbal formula Astragali Radix and Rehmanniae Radix exerted wound healing effect on human skin fibroblast cell line Hs27 via the activation of transformation growth factor (TGF-β) pathway and ..." in: Phytomedicine : international journal of phytotherapy and phytopharmacology, Vol. 20, Issue 1, pp. 9-16, (2013) (PubMed).

Liu, Liu, Lin, Liu, Hou, Hao, Liu, Zhang, Iwamori: "Lewis y regulate cell cycle related factors in ovarian carcinoma cell RMG-I in vitro via ERK and Akt signaling pathways." in: International journal of molecular sciences, Vol. 13, Issue 1, pp. 828-39, (2012) (PubMed).

Miura, Kirino, Endo, Morisaka, Kuroda, Takagi, Ueda: "Tracing putative trafficking of the glycolytic enzyme enolase via SNARE-driven unconventional secretion." in: Eukaryotic cell, Vol. 11, Issue 8, pp. 1075-82, (2012) (PubMed).

Target Details for Cyclin D1

Target: Cyclin D1 (CCND1)

Alternative Name: Cyclin D1 (CCND1 Products)

Synonyms: BCL1 antibody, D11S287E antibody, PRAD1 antibody, U21B31 antibody, AI327039 antibody, CycD1 antibody, Cyl-1 antibody, bcl-1 antibody, cD1 antibody, cb161 antibody, cycd1 antibody, etID37810.7 antibody, fb52e01 antibody, fc45c08 antibody, fc83a12 antibody, id:ibd1198 antibody, wu:fb52e01 antibody, wu:fc45c08 antibody, wu:fc83a12 antibody, ccnd1b antibody, prad1 antibody, ccnd1 antibody, ccnd1a antibody, bcl1 antibody, d11s287e antibody, u21b31 antibody, CCND1 antibody, cyclin D1 antibody, cyclin D1 S homeolog antibody, cyclin D1 L homeolog antibody, CCND1 antibody, Ccnd1 antibody, ccnd1 antibody, ccnd1.S antibody, ccnd1.L antibody

Background: Considerable effort directed towards the identification of G1 cyclins has led to the isolation of cyclin D, cyclin C and cyclin E. Of these, cyclin D corresponds to a putative human oncogene, designated PRAD1, which maps at the site of the Bcl1 rearrangement in certain lymphomas and leukemias. Two additional human type D cyclins, as well as their mouse homologs, have been identified. Evidence has established that members of the cyclin D family function to regulate phosphorylation of the retinoblastoma gene product, thereby activating E2F transcription factors.Synonyms: BCL-1 oncogene, BCL1, CCND1, Cyclin-D1, G1/S-specific cyclin-D1, PRAD-1 oncogene, PRAD1

Molecular Weight: approx. 36 kDa

Gene ID: 595

NCBI Accession: NP_444284

UniProt: P24385

Pathways: PI3K-Akt Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, ER-Nucleus Signaling