ARHGEF2 antibody

Catalog No. ABIN498403

Product Details anti-ARHGEF2 Antibody

Target: ARHGEF2 (rho/rac Guanine Nucleotide Exchange Factor (GEF) 2 (ARHGEF2))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ARHGEF2 antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: This antibody detects endogenous levels of ARHGEF2 protein. (region surrounding Pro881)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Purity: > 95 % by SDS-PAGE

Application Details

Application Notes: Western Blot: 1/500 - 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for ARHGEF2

Target: ARHGEF2 (rho/rac Guanine Nucleotide Exchange Factor (GEF) 2 (ARHGEF2))

Alternative Name: ARHGEF2 (ARHGEF2 Products)

Synonyms: MGC82333 antibody, MGC86214 antibody, MGC75855 antibody, AA408978 antibody, GEF antibody, GEF-H1 antibody, GEFH1 antibody, LFP40 antibody, Lbcl1 antibody, Lfc antibody, P40 antibody, mKIAA0651 antibody, GEF-H1/LFC antibody, Rho/Rac guanine nucleotide exchange factor 2 antibody, Rho/Rac guanine nucleotide exchange factor 2 S homeolog antibody, rho/rac guanine nucleotide exchange factor (GEF) 2 antibody, ARHGEF2 antibody, arhgef2.S antibody, arhgef2 antibody, Arhgef2 antibody

Background: GEFs catalyze the release of GDP from inactive Ras proteins to ena-ble GTP binding. Lfc (also designated Rho/Rac guanine nucleotide exchange factor 2, lymphoid blast crisis-like 1, ARHGEF2, GEF-H1, KIAA0651 or LFP40) is an 894 amino acid GEF for Rho A, a member of the Ras family that regulates the Actin cytoskeleton during the formation of stress fibers. Lfc directly interacts with Cingulin, a junctional adapter that inhibits Rho A, thus preventing phorbol-12-myristate-13-acetate-induced contraction and apoptosis. Mutations in the p53 gene transcriptionally activate Lfc, thereby increasing tumor cell proliferation.Synonyms: GEF-H1, Guanine nucleotide exchange factor H1, KIAA0651, LFP40, Microtubule-regulated Rho-GEF, Proliferating cell nucleolar antigen p40, Rho guanine nucleotide exchange factor 2

Molecular Weight: approx. 110 kDa

Gene ID: 9181

NCBI Accession: NP_004714

UniProt: Q92974

Pathways: Negative Regulation of intrinsic apoptotic Signaling