ZNF148 antibody

Catalog No. ABIN498436

Product Details anti-ZNF148 Antibody

Target: ZNF148 (Zinc Finger Protein 148 (ZNF148))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ZNF148 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody detects endogenous levels of ZBP-89 protein. (region surrounding Glu84)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Purity: > 95 % by SDS-PAGE

Application Details

Application Notes: Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200. Immunofluorescence: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for ZNF148

Target: ZNF148 (Zinc Finger Protein 148 (ZNF148))

Alternative Name: ZNF148 (ZNF148 Products)

Synonyms: BERF-1 antibody, BFCOL1 antibody, HT-BETA antibody, ZBP-89 antibody, ZFP148 antibody, pHZ-52 antibody, 2210405J08Rik antibody, AI480666 antibody, AW045217 antibody, Znf148 antibody, Zbp-89 antibody, fb06h05 antibody, wu:fb06h05 antibody, zfp149 antibody, zgc:165342 antibody, SLC12A8 antibody, zinc finger protein 148 antibody, ZNF148 antibody, Zfp148 antibody, znf148 antibody

Background: ZBP-89, also known as BFCOL1, BERF1 and ZNF 148, is a zinc finger transcription factor that is universally expressed. ZBP-89, a Kruppel-like repressor protein, is the silencer element binding factor for Vimentin. ZBP-89 has been shown to bind to GC-rich DNA elements in promoters for gastrin, ornithine decarboxylase and the cyclin-dependent kinase inhibitor p21 (also designated Cip1 or WAF1). ZBP-89 expression is induced by trans-retinoic acid or butyrate, which also induces terminal differentiation of colon cancer cells. ZBP-89 cooperates with histone acetyltransferase coactivator p300 in the regulation of p21, a cyclin-dependent kinase inhibitor whose associated gene is a target gene of p53. ZBP-89 also regulates cell proliferation, in part, through its ability to directly bind the p53 protein and retard its nuclear export. Elevated levels of ZBP-89 induce growth arrest and apoptosis in human gastrointestinal cells.Synonyms: Transcription factor ZBP-89, ZBP89, Zinc finger DNA-binding protein 89, Zinc finger protein 148

Molecular Weight: approx. 89 kDa

Gene ID: 7707

NCBI Accession: NP_068799

UniProt: Q9UQR1