BNIP2 antibody

Catalog No. ABIN498505

Product Details anti-BNIP2 Antibody

Target: BNIP2 (BCL2/adenovirus E1B 19kDa Interacting Protein 2 (BNIP2))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BNIP2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody detects endogenous levels of BNIP-2 protein. (region surrounding Glu122)

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse.

Purification: Affinity Chromatography using epitope-specific immunogen.

Application Details

Application Notes: Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH ~7.2, 0.05 % Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for BNIP2

Target: BNIP2 (BCL2/adenovirus E1B 19kDa Interacting Protein 2 (BNIP2))

Alternative Name: BNIP2 (BNIP2 Products)

Synonyms: BNIP-2 antibody, NIP2 antibody, 5730523P12Rik antibody, BNIP2beta antibody, Nip21 antibody, BCL2 interacting protein 2 antibody, BCL2/adenovirus E1B interacting protein 2 antibody, BNIP2 antibody, Bnip2 antibody

Background: The adenovirus E1B protein is a viral homolog of the Bcl-2 family of proteins that are involved in regulating cell death. A family of interacting proteins, which are designated Nip or Bnip and include BNIP-1, BNIP-2, BNIP-3 and Nix, associate with both the E1B protein and Bcl-2 proteins to mediate apoptotic signaling. BNIP-1 contains a hydrophobic transmembrane domain, which enables its localization to the nuclear envelope, endoplasmic recticulum and mitochondria. BNIP-2, (previously designated Nip2 and Nip21 in human and mouse respectively), shares homology with the non-catalytic domain of Cdc42 GTPase-activating protein (Cdc42GAP). Through binding to Cdc42GAP, BNIP-2 enhances the GTPase activity of Cdc42GAP, facilitating the hydrolysis of GTP bound to Cdc42 and thereby, mediating the signaling pathways involving receptor kinases, small GTPases and apoptotic proteins. Nix, which is also designated Nip3L or Bnip3L, is highly related to BNIP-3, and both proteins localize to the mitochondria where they associate with Bcl-2 proteins.Synonyms: BCL2/adenovirus E1B 19 kDa protein-interacting protein 2, NIP2

Molecular Weight: approx. 34 kDa

Gene ID: 663

NCBI Accession: NP_004321

UniProt: Q12982

Pathways: Regulation of Muscle Cell Differentiation