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BNIP3 antibody

BNIP3 Reactivity: Human, Mouse, Rat IHC (p) Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN498556
  • Target See all BNIP3 Antibodies
    BNIP3 (BCL2/adenovirus E1B 19kDa Interacting Protein 3 (BNIP3))
    Reactivity
    • 111
    • 46
    • 30
    • 1
    • 1
    Human, Mouse, Rat
    Host
    • 99
    • 9
    • 7
    Rabbit
    Clonality
    • 97
    • 18
    Polyclonal
    Conjugate
    • 44
    • 7
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    This BNIP3 antibody is un-conjugated
    Application
    • 69
    • 31
    • 29
    • 27
    • 25
    • 20
    • 14
    • 12
    • 9
    • 4
    • 3
    • 3
    • 1
    • 1
    Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Specificity
    This antibody detects endogenous levels of BNIP-3 protein. (region surrounding Glu96)
    Cross-Reactivity (Details)
    Species reactivity (expected):Mouse and Rat.
    Species reactivity (tested):Human.
    Purification
    Affinity chromatography using epitope-specific immunogen
    Purity
    > 95 % (by SDS-PAGE)
    Top Product
    Discover our top product BNIP3 Primary Antibody
  • Application Notes
    Immunohistochemistry on paraffin sections: 1: 50 - 1: 200.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Concentration
    1.0 mg/mL
    Buffer
    Phosphate buffered saline (PBS), pH 7.2, 0.05 % sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.
  • Target
    BNIP3 (BCL2/adenovirus E1B 19kDa Interacting Protein 3 (BNIP3))
    Alternative Name
    NIP3 (BNIP3 Products)
    Synonyms
    NIP3 antibody, Nip3 antibody, BCL2 interacting protein 3 antibody, BCL2/adenovirus E1B interacting protein 3 antibody, BNIP3 antibody, Bnip3 antibody
    Background
    BNIP3, formerly NIP3 (nineteen kDa interacting protein-3), is a pro-apoptotic, mitochondrial protein classified in the Bcl 2 family based on limited sequence homology to the Bcl 2 homology 3 (BH3) domain (amino acids 110-118) and C-terminal TM domain. BNIP3 expressed in yeast and mammalian cells interacts with survival promoting proteins Bcl 2, Bcl XL, CED9 and the adenovirus E1B 19K protein. Typically the BH3 domain of pro-apoptotic Bcl-2 homologues mediates Bcl 2/Bcl XL heterodimerization and confers pro-apoptotic activity. BNIP3 represents a subfamily of Bcl 2 related proteins, which functions without a typical BH3 domain to regulate apoptosis from both mitochondrial and nonmitochondrial sites by selective Bcl 2/Bcl XL interactions. The N-terminus (residues 1-49) and the C-terminus TM domain of BNIP3 are critical for Bcl 2 heterodimerization, and either region is sufficient for Bcl XL interaction. The TM domain of BNIP3 is critical for homodimerization, pro-apoptotic function, and mitochondrial targeting. BNIP3 contains PEST sequences suggesting that the protein may be susceptible to rapid degradation by proteases. PEST sequences commonly contain high local concentrations of amino acids P, E, S, T, and D flanked by charged amino acids and these are abundantly present in NIP3. Thus, the posttranslational control of BNIP3 expression through rapid protein degradation may constitute a mechanism for regulating the intracellular levels of a potentially lethal protein.Synonyms: BCL2/adenovirus E1B 19 kDa protein-interacting protein 3, BNIP3
    Molecular Weight
    approx. 22 kDa
    Gene ID
    664
    NCBI Accession
    NP_004043
    UniProt
    Q12983
    Pathways
    Autophagy, Brown Fat Cell Differentiation
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