NME1 antibody
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- Target See all NME1 Antibodies
- NME1 (Non-Metastatic Cells 1, Protein (NM23A) Expressed in (NME1))
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Reactivity
- Human, Mouse, Rat
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- This NME1 antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
- Specificity
- This antibody detects endogenous levels of NM23-H1 protein. (region surrounding Tyr52)
- Cross-Reactivity (Details)
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Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human. - Purification
- Affinity chromatography
- Purity
- > 95 % by SDS-PAGE
- Top Product
- Discover our top product NME1 Primary Antibody
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- Application Notes
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Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Concentration
- 1,0 mg/mL
- Buffer
- Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- Avoid repeated freezing and thawing.
- Storage
- 4 °C/-20 °C
- Storage Comment
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
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- Target
- NME1 (Non-Metastatic Cells 1, Protein (NM23A) Expressed in (NME1))
- Alternative Name
- NDP Kinase A (NME1 Products)
- Synonyms
- AWD antibody, GAAD antibody, NB antibody, NBS antibody, NDKA antibody, NDPK-A antibody, NDPKA antibody, NM23 antibody, NM23-H1 antibody, AL024257 antibody, NM23-M1 antibody, NM23A antibody, NDPK-Z1 antibody, NM23-B antibody, NM23-Z1 antibody, ndpkz1 antibody, nm23b antibody, nme1 antibody, nme2 antibody, nme2b1 antibody, NME1 antibody, NME1-NME2 antibody, NM23-C1 antibody, ndpk-b antibody, ndpkb antibody, nm23-h2 antibody, nm23ndk antibody, puf antibody, NBR-A antibody, NBR-B antibody, NME1-1 antibody, nm23ndk-a antibody, NDK I antibody, NDPK I antibody, T30A10.80 antibody, T30A10_80 antibody, NDP kinase I antibody, NME/NM23 nucleoside diphosphate kinase 1 antibody, NME/NM23 nucleoside diphosphate kinase 2b, tandem duplicate 1 antibody, non-metastatic cells 1, protein (NM23A) expressed in antibody, NME/NM23 nucleoside diphosphate kinase 2 S homeolog antibody, NME/NM23 nucleoside diphosphate kinase 2 L homeolog antibody, nucleoside diphosphate kinase antibody, nucleoside diphosphate kinase 1 antibody, NME1 antibody, Nme1 antibody, nme2b.1 antibody, nme2.S antibody, nme2.L antibody, LOC547870 antibody, NDPK1 antibody, LOC107790563 antibody
- Background
- The nm23 gene, a potential suppressor of metastasis, was originally identified by differential hybridization between two murine melanoma sub-lines, one with a high and the second with a low metastatic capacity. Highly metastatic sublines exhibit much lower levels of nm23 than less metastatic cells. Based on sequence analysis, nm23 appears highly related to nucleotide diphosphate kinases (NDP). In humans, NDP kinases A and B are identical to two isotypes of human nm23 homologs, namely nm23-H1 and -H2, respectively. nm23-H2 is identical in sequence to PuF, a transcription factor that binds to nucleasehypersensitive elements at positions 142 to 115 of the human c-Myc promotor.Synonyms: GAAD, Granzyme A-activated DNase, NDPKA, NM23, NME1, Nucleoside diphosphate kinase A, nm23-H1
- Molecular Weight
- approx. 20 kDa
- Gene ID
- 4830
- NCBI Accession
- NP_000260
- UniProt
- P15531
- Pathways
- Apoptosis, Nucleotide Phosphorylation, Carbohydrate Homeostasis, Ribonucleoside Biosynthetic Process
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