DAB1 antibody

Catalog No. ABIN498639

Product Details anti-DAB1 Antibody

Target: DAB1 (Disabled Homolog 1 (Drosophila) (DAB1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DAB1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Specificity: This antibody detects endogenous levels of Dab1 protein. (region surrounding Glu214)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Purity: > 95 % by SDS-PAGE

Application Details

Application Notes: Western Blot: 1/500 - 1/1000. Immunofluorescence: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for DAB1

Target: DAB1 (Disabled Homolog 1 (Drosophila) (DAB1))

Alternative Name: DAB1 (DAB1 Products)

Synonyms: AI956902 antibody, C630028C02Rik antibody, scm antibody, scr antibody, scrambler antibody, yot antibody, disabled-1 antibody, LOC100232823 antibody, si:dkey-242m13.1 antibody, DAB1 antibody, zgc:136547 antibody, DAB1, reelin adaptor protein antibody, disabled 1 antibody, disabled homolog 1 antibody, Dab, reelin signal transducer, homolog 1a (Drosophila) antibody, Dab, reelin signal transducer, homolog 1b (Drosophila) antibody, DAB1 antibody, Dab1 antibody, LOC100232823 antibody, dab1a antibody, dab1b antibody

Background: Dab1, a homolog of the Drosophila Disabled protein, is an adaptor protein involved in neural development. This cytoplasmic protein is tyrosine-phosphorylated during rapid expansion of the developing nervous system, and it is thought to interact with other proteins via a domain similar to the PTB domains of the Shc family. Dab1 has been shown to interact with the SH2 domains of Src, Fyn and Abl. Mutations in Dab1 result in widespread abnormalities in the brain, similar to those seen in Reelin mutants. Reelin is a secreted protein thought to play a role in directing migrating neurons. Evidence suggests that Dab1 functions downstream of Reelin in a signaling pathway involved in positioning cells in the developing brain. Dab2 (also designated DOC-2) is a mitogen-responsive phosphoprotein that binds the SH3 domain of Grb2, and it is thought to be a negative regulator of growth.Synonyms: Disabled homolog 1

Molecular Weight: approx. 60 kDa

Gene ID: 1600

NCBI Accession: NP_066566

UniProt: O75553