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GUCY1A1 antibody

GUCY1A1 Reactivity: Human, Rat, Mouse WB, IHC (p) Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN498662
  • Target See all GUCY1A1 Antibodies
    GUCY1A1 (Guanylate Cyclase 1 Soluble Subunit Alpha (GUCY1A1))
    Reactivity
    • 37
    • 29
    • 14
    • 1
    • 1
    • 1
    Human, Rat, Mouse
    Host
    • 45
    • 7
    Rabbit
    Clonality
    • 47
    • 5
    Polyclonal
    Conjugate
    • 26
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    This GUCY1A1 antibody is un-conjugated
    Application
    • 39
    • 20
    • 13
    • 13
    • 13
    • 8
    • 3
    • 3
    • 3
    • 3
    • 2
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Specificity
    This antibody detects endogenous levels of GCS-alpha-1 protein. (region surrounding Asp423)
    Cross-Reactivity (Details)
    Species reactivity (expected):Mouse and Rat.
    Species reactivity (tested):Human.
    Purification
    Affinity chromatography
    Purity
    > 95 % by SDS-PAGE
    Top Product
    Discover our top product GUCY1A1 Primary Antibody
  • Application Notes
    Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Concentration
    1,0 mg/mL
    Buffer
    Phosphate buffered saline (PBS), pH ~7.2, 15 mM Sodium Azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Target
    GUCY1A1 (Guanylate Cyclase 1 Soluble Subunit Alpha (GUCY1A1))
    Alternative Name
    Guanylate Cyclase Soluble GUCY1A3 (GUCY1A1 Products)
    Synonyms
    GC-SA3 antibody, GUC1A3 antibody, GUCA3 antibody, GUCSA3 antibody, GUCY1A1 antibody, 1200016O07Rik antibody, Gucy1a1 antibody, SGC antibody, im:7136149 antibody, sgc1a1 antibody, zgc:112159 antibody, gucy1a3 antibody, guanylate cyclase 1 soluble subunit alpha antibody, guanylate cyclase 1, soluble, alpha 3 antibody, guanylate cyclase 1 soluble subunit alpha 3 antibody, soluble guanylyl cyclase alpha subunit antibody, GUCY1A3 antibody, Gucy1a3 antibody, gucy1a3 antibody
    Background
    Guanylate cyclases belong to the adenylyl cyclase class-4/guanylyl cyclase family. There are two forms of guanylate cyclase. The soluble form, known as GCS or sGC, act as receptors for nitric oxide. The membrane-bound receptor form, known as GC, are peptide hormone receptors. GCS is a cGMP-synthesizing enzyme, which is the major receptor for the neurotransmitter nitric oxide (NO). It plays a crucial role in smooth muscle contractility, platelet reactivity and neurotransmission. GCS is a heme containing heterodimer, consisting of one 72 kDa alpha subunit and one 65 kDa beta subunit. The heme moeity mediates NO activation, and this heme group also binds carbon monoxide, which weakly stimulates the enzyme. Both NO and CO stimulation are enhanced by the allosteric activator 3-(5'-hydroxymethyl-2'furyl)-benzylindazole, YC-1. YC-1 can also stimulate GCS in a NOindependent manner. Both alpha and beta subunits are required for cGMP generation, and at least two isoforms exist for each subunit. Heterodimers consisting of alpha-1/beta-1 and alpha-2/beta-1 have been identified, and both display similar enzymatic activity.Synonyms: GCS-alpha-1, GCS-alpha-3, GUC1A3, GUCSA3, GUCY1A1, Guanylate cyclase soluble subunit alpha-3, Soluble guanylate cyclase large subunit, sGC alpha
    Molecular Weight
    approx. 77 kDa
    Gene ID
    2982
    NCBI Accession
    NP_000847
    UniProt
    Q02108
    Pathways
    Myometrial Relaxation and Contraction
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