TRAF6 antibody

Catalog No. ABIN498675

Product Details anti-TRAF6 Antibody

Target: TRAF6 (TNF Receptor-Associated Factor 6 (TRAF6))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This TRAF6 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody detects endogenous levels of Mfn2 protein. (region surrounding His154)

Cross-Reactivity (Details): Species reactivity (expected):Mouse and Rat.
Species reactivity (tested):Human.

Purification: Affinity chromatography

Purity: > 95 % by SDS-PAGE

Application Details

Application Notes: Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for TRAF6

Target: TRAF6 (TNF Receptor-Associated Factor 6 (TRAF6))

Alternative Name: TRAF6 / RNF85 (TRAF6 Products)

Synonyms: MGC:3310 antibody, RNF85 antibody, 2310003F17Rik antibody, AI851288 antibody, C630032O20Rik antibody, rnf85 antibody, traf6 antibody, xtraf6 antibody, CG10961 antibody, DTRAF2 antibody, Dmel\\CG10961 antibody, TRAF antibody, TRAF2 antibody, TRAF6 antibody, Traf2 antibody, dTRAF2 antibody, dTRAF6 antibody, dTraf2 antibody, dtraf2 antibody, GB10539 antibody, fi15f02 antibody, wu:fi15f02 antibody, zgc:63704 antibody, TNF receptor associated factor 6 antibody, TNF receptor-associated factor 6 antibody, TNF receptor associated factor 6 L homeolog antibody, TNF-receptor-associated factor 6 antibody, TNF receptor associated factor 6 S homeolog antibody, TRAF6 antibody, Traf6 antibody, traf6.L antibody, traf6 antibody, LOC551819 antibody, traf6.S antibody

Background: Tumor necrosis factor receptor-associated factor 6 (TRAF6) regulates adaptive immunity, innate immunity and bone metabolism. TRAF6 is a ubiquitin (Ub) ligase that mediates the activation of protein kinases, such as transforming growth factor beta-activated kinase (TAK1) and IkappaB kinase (IKK), by catalyzing the formation of a unique polyubiquitin chain linked through Lys 63 of Ub. TRAF6 is essential for activating NFκB signaling pathway in response to interleukin-1 and Toll-like receptor ligands. The coiled-coil domain of TRAF6 is essential for its auto-ubiquitination and activating NFkappaB signaling pathway. TRAF6 interacts with various protein kinases including IRAK1/IRAK, SRC and PKCzeta, which provides a link between distinct signaling pathways.Synonyms: Interleukin-1 signal transducer, RING finger protein 85, TNF receptor-associated factor 6

Molecular Weight: approx. 58 kDa

Gene ID: 7189

NCBI Accession: NP_004611

UniProt: Q9Y4K3

Pathways: NF-kappaB Signaling, TCR Signaling, TLR Signaling, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response, Tube Formation, Hepatitis C, Toll-Like Receptors Cascades, Ubiquitin Proteasome Pathway