Cyclin E2 antibody

Catalog No. ABIN498694

Product Details anti-Cyclin E2 Antibody

Target: Cyclin E2 (CCNE2)

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cyclin E2 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody detects endogenous levels of Cyclin E2 protein. (region surrounding asn387)

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse.

Purification: Affinity chromatography

Purity: > 95 % by SDS-PAGE

Application Details

Application Notes: Western Blot: 1/500 - 1/1000. Immunohistochemistry 1/50 - 1/200. Immunofluorescence: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Cyclin E2

Target: Cyclin E2 (CCNE2)

Alternative Name: Cyclin E2 (CCNE2 Products)

Synonyms: ccne2 antibody, MGC53684 antibody, MGC132360 antibody, cb165 antibody, sb:cb165 antibody, zgc:86724 antibody, CCNE2 antibody, cyce2 antibody, CYCE2 antibody, cyclin E2 antibody, cyclin E2 L homeolog antibody, cyclin E1 L homeolog antibody, CCNE2 antibody, ccne2.L antibody, ccne2 antibody, ccne1.L antibody, Ccne2 antibody

Background: Cyclin E, along with the three cyclin D proteins and cyclin C, has been shown to represent a putative G1 cyclin on the basis of its cyclic pattern of mRNA expression, with maximal levels being detected near the G1/S boundary. cyclin E has been found to be associated with the transcription factor E2F in a temporally regulated manner. Cyclin E2 is a cyclin E-related protein that specifically interacts with Cdk2 and Cdk3 and with p27 and p21. Cyclin E2 expression peaks at the G1/S phase transition of the cell cycle, in parallel with cyclin E. Whereas cyclin E1 is expressed in most proliferating normal and tumor cells, cyclin E2 levels are low or undetectable in nontransformed cells, and are elevated in tumor-derived cells.Synonyms: CCNE2, Cyclin-E2, G1/S-specific cyclin-E2

Molecular Weight: approx. 46 kDa

Gene ID: 9134

NCBI Accession: NP_477097

UniProt: O96020

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases