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Connexin 43/GJA1 antibody (pSer367)

GJA1 Reactivity: Human, Rat, Mouse WB, IHC (p) Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN498754
  • Target See all Connexin 43/GJA1 (GJA1) Antibodies
    Connexin 43/GJA1 (GJA1) (Gap Junction Protein, alpha 1, 43kDa (GJA1))
    Binding Specificity
    • 21
    • 16
    • 15
    • 12
    • 8
    • 7
    • 7
    • 7
    • 7
    • 4
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    pSer367
    Reactivity
    • 135
    • 93
    • 91
    • 33
    • 12
    • 12
    • 11
    • 10
    • 10
    • 8
    • 7
    • 6
    • 6
    • 4
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Rat, Mouse
    Host
    • 130
    • 8
    • 4
    • 1
    Rabbit
    Clonality
    • 134
    • 8
    Polyclonal
    Conjugate
    • 83
    • 7
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    This Connexin 43/GJA1 antibody is un-conjugated
    Application
    • 98
    • 54
    • 48
    • 29
    • 21
    • 16
    • 13
    • 13
    • 11
    • 9
    • 8
    • 5
    • 3
    • 2
    • 1
    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Specificity
    This antibody detects endogenous levels of Connexin 43 protein when phosphorylated at Ser367.
    Cross-Reactivity (Details)
    Species reactivity (expected):Mouse and Rat.
    Species reactivity (tested):Human.
    Purification
    Affinity chromatography
    Purity
    > 95 % by SDS-PAGE
    Top Product
    Discover our top product GJA1 Primary Antibody
  • Application Notes
    Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Concentration
    1,0 mg/mL
    Buffer
    Phosphate buffered saline (PBS), pH 7.2., 15 mM sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Target
    Connexin 43/GJA1 (GJA1) (Gap Junction Protein, alpha 1, 43kDa (GJA1))
    Alternative Name
    GJA1 / Cx43 (GJA1 Products)
    Synonyms
    AVSD3 antibody, CX43 antibody, DFNB38 antibody, GJAL antibody, HLHS1 antibody, HSS antibody, ODDD antibody, Cx43 antibody, AU042049 antibody, AW546267 antibody, Cnx43 antibody, Cx43alpha1 antibody, Gja-1 antibody, Npm1 antibody, connexin43 antibody, cx43a1 antibody, gja1 antibody, zfCx43.3 antibody, connexin 43 antibody, gja1-A antibody, cx43 antibody, gjal antibody, oddd antibody, dfnb38 antibody, gap junction protein alpha 1 antibody, gap junction protein, alpha 1 antibody, connexin 43 antibody, gap junction protein alpha 1 L homeolog antibody, CONNEXIN 43 protein antibody, GJA1 antibody, Gja1 antibody, cx43 antibody, gja1.L antibody, gja1 antibody, CONNEXIN 43 antibody
    Background
    The connexins are a group of GAP junction proteins, which form a hexamer to compose a connexon. Clusters of connexons form a GAP junction through which low molecular weight proteins may diffuse from cell to cell. Several mammalian cells with malignant phenotypes exhibit decreased connexin expression and GAP junction communication. There is a decrease in GAP junctional communication in Src transformed cells. The decreased communication appears to be associated with tyrosine phosphorylation of connexin 43. Activated c-Src phosphorylates the C-terminal tail of connexin 43 on residue Tyr 265, resulting in a stable interaction between both proteins leading to inhibition of GAP junctional communication. In addition to tyrosine phosphorylation, connexin 43 has also been shown to be phosphorylated on serine in the absence of Src kinases and on both serine and tyrosine in cells expressing Src kinases such as pp60v-Src and/or c-Src.Synonyms: Connexin-43, GJAL, Gap junction 43 kDa heart protein, Gap junction alpha-1 protein
    Molecular Weight
    approx. 43 kDa
    Gene ID
    2697
    NCBI Accession
    NP_000156
    UniProt
    P17302
    Pathways
    MAPK Signaling, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization
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