HLA-DOA antibody

Catalog No. ABIN498962

Product Details anti-HLA-DOA Antibody

Target: HLA-DOA (HLA Class II DO alpha (HLA-DOA))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HLA-DOA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Specificity: This antibody detects endogenous levels of HLADOalpha protein. (region surrounding Val111)

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Affinity chromatography

Purity: > 95 % by SDS-PAGE

Application Details

Application Notes: Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for HLA-DOA

Target: HLA-DOA (HLA Class II DO alpha (HLA-DOA))

Alternative Name: HLA Class II DO alpha / HLA-DOA (HLA-DOA Products)

Synonyms: HLA-DNA antibody, HLA-DZA antibody, HLADZ antibody, major histocompatibility complex, class II, DO alpha antibody, HLA-DOA antibody

Background: Reacts with HLA-DO, a heterodimer formed by DNalpha and DObeta subunits in B cells. DNalpha and DObeta are the products of the non-classical class II genes, HLA-DNA and HLA-DOB, respectively. DO forms tight complexes with DM in the endoplasmic reticulum and is thereby sorted to lysosomal vesicles during antigen processing and presentation. It is tightly associated with DM and it is selectively expressed on antigenpresenting cells, such as B cells, dendritic cells and thymic epithelial cells. DO can enhance the efficiency of peptide loading and has been found to stabilize DM at low pH , preserving its chaperon activity. DO-DM complexes are more efficient than DM in protecting empty DR molecules. Reports describe DO as a co-chaperone of DM. This antibody has also been reported to be suitable for western blot and immunoprecipitation.Synonyms: HLA class II histocompatibility antigen DO alpha chain, HLA-DNA, HLA-DZA, MHC DN-alpha, MHC DZ alpha, MHC class II antigen DOA

Molecular Weight: approx. 34 kDa

Gene ID: 3111

NCBI Accession: NP_002110

UniProt: P06340