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p53 antibody

TP53 Reactivity: Human, Mouse, Rat WB, IHC (p) Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN499004
  • Target See all p53 (TP53) Antibodies
    p53 (TP53) (Tumor Protein P53 (TP53))
    Reactivity
    • 890
    • 270
    • 220
    • 110
    • 45
    • 34
    • 31
    • 18
    • 10
    • 8
    • 8
    • 6
    • 4
    • 4
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Human, Mouse, Rat
    Host
    • 678
    • 228
    • 6
    • 2
    • 1
    • 1
    Rabbit
    Clonality
    • 612
    • 303
    • 1
    Polyclonal
    Conjugate
    • 511
    • 49
    • 39
    • 26
    • 25
    • 24
    • 24
    • 24
    • 22
    • 22
    • 22
    • 13
    • 13
    • 13
    • 13
    • 13
    • 13
    • 5
    • 5
    • 5
    • 5
    • 5
    • 5
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This p53 antibody is un-conjugated
    Application
    • 720
    • 336
    • 254
    • 216
    • 208
    • 150
    • 124
    • 123
    • 119
    • 62
    • 61
    • 32
    • 20
    • 17
    • 13
    • 12
    • 12
    • 10
    • 9
    • 8
    • 6
    • 4
    • 3
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
    Specificity
    This antibody detects endogenous levels of p53 protein. (region surrounding Ser371)
    Cross-Reactivity (Details)
    Species reactivity (expected):Mouse and Rat.
    Species reactivity (tested):Human.
    Purification
    Affinity chromatography
    Purity
    > 95 % by SDS-PAGE
    Top Product
    Discover our top product TP53 Primary Antibody
  • Application Notes
    Western Blot: 1/500 - 1/1000. Immunohistochemistry: 1/50 - 1/200.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Concentration
    1,0 mg/mL
    Buffer
    Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Target
    p53 (TP53) (Tumor Protein P53 (TP53))
    Alternative Name
    p53 (TP53) (TP53 Products)
    Synonyms
    BCC7 antibody, LFS1 antibody, P53 antibody, TRP53 antibody, p53 antibody, Trp53 antibody, brp53 antibody, drp53 antibody, etID22686.5 antibody, fb40d06 antibody, wu:fb40d06 antibody, zgc:111919 antibody, Tp53 antibody, bbl antibody, bfy antibody, bhy antibody, p44 antibody, tp53 antibody, Xp53 antibody, TP53 antibody, tumor protein p53 antibody, cellular tumor antigen p53 antibody, transformation related protein 53 antibody, tumor protein p53 L homeolog antibody, TP53 antibody, CpipJ_CPIJ002758 antibody, Tp53 antibody, tp53 antibody, Trp53 antibody, tp53.L antibody
    Background
    P53 is a DNA-binding, oligomerization domain and transcription activation domain-containing tumor suppressor that upregulates growth arrest and apoptosis-related genes in response to stress signals, thereby influencing programmed cell death, cell differentiation and cell cycle control mechanisms. p53 localizes to the nucleus yet can be chaperoned to the cytoplasm by the negative regulator MDM2, an E3 ubiquitin ligase that is upregulated in the presence of active p53, where MDM2 polyubiquitinates p53 for proteasome targeting. p53 can assemble into tetramers in the absence of DNA, fluctuates between latent and active (DNA-binding) conformations, and is differentially activated through posttranslational modifications including phosphorylation and acetylation. Mutations in the DNA-binding domain (DBD) (amino acids 110-286) of p53 can compromise energetically favorable association with cis elements and are implicated in several human cancers.Synonyms: Cellular tumor antigen p53, NY-CO-13, Phosphoprotein p53, Tumor suppressor p53
    Molecular Weight
    approx. 53, 43 kDa
    Gene ID
    7157
    NCBI Accession
    NP_000537
    UniProt
    P04637
    Pathways
    p53 Signaling, MAPK Signaling, PI3K-Akt Signaling, Apoptosis, AMPK Signaling, Chromatin Binding, ER-Nucleus Signaling, Positive Regulation of Endopeptidase Activity, Hepatitis C, Protein targeting to Nucleus, Autophagy, Warburg Effect
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