DHPS antibody

Catalog No. ABIN499058

Product Details anti-DHPS Antibody

Target: DHPS (Deoxyhypusine Synthase (DHPS))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DHPS antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: This antibody detects endogenous levels of DHS protein. (region surrounding Leu87)

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Affinity chromatography

Purity: > 95 % by SDS-PAGE

Application Details

Application Notes: Western Blot: 1/500 - 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1,0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for DHPS

Target: DHPS (Deoxyhypusine Synthase (DHPS))

Abstract: DHPS Products

Synonyms: MGC76269 antibody, zgc:56018 antibody, zgc:77325 antibody, wu:fb52g04 antibody, wu:fj48f12 antibody, zgc:111900 antibody, MGC82178 antibody, DDBDRAFT_0186679 antibody, DDBDRAFT_0266388 antibody, DDB_0186679 antibody, DDB_0266388 antibody, DHS antibody, DS antibody, MIG13 antibody, Dhs antibody, DYS antibody, EDA22 antibody, K18J17.7 antibody, K18J17_7 antibody, deoxyhypusine synthase antibody, embryo sac development arrest 22 antibody, deoxyhypusine synthase antibody, deoxyhypusine synthase S homeolog antibody, Deoxyhypusine synthase antibody, DHPS antibody, dhps antibody, dhps.S antibody, SHELL_RS05250 antibody, PF14_0125 antibody, CNJ02480 antibody, Tb10.70.4900 antibody, Tb927.1.870 antibody, MMAH_RS09430 antibody, TAGG_RS03310 antibody, TAGG_RS05175 antibody, Mesil_2448 antibody, Trad_0088 antibody, Igag_1842 antibody, Desac_0724 antibody, MZHIL_RS05480 antibody, MZHIL_RS05835 antibody, MZHIL_RS06250 antibody, Dhps antibody, DHS antibody

Background: Deoxyhypusine synthase (DHS) is crucial for the posttranslational formation of hypusine, a modification of a specific lysine residue in eukaryotic initiation factor 5A (eIF-5A). Hypusine is formed by posttranslational modifications involving two enzymatic steps catalyzed by DHS and deoxyhypusine hydroxylase (DOHH). eIF-5A is essential for eukaryotic cell proliferation. DHS, which belongs to the deoxyhypusine synthase family of proteins, is important for the first step in the hypusine biosynthesis pathway. It acts as a catalyst for the NAD-dependent oxidative cleavage of spermidine and the ensuing transfer of the butylamine moiety of spermidine to the eIF-5A protein, to create the intermediate deoxyhypusine residue.

Molecular Weight: approx. 45 kDa

Gene ID: 1725

NCBI Accession: NP_001921

UniProt: P49366