Deoxyhypusine Synthase (DHPS) antibody

Details for Product No. ABIN499058, Supplier: Log in to see
Antigen
  • MGC76269
  • zgc:56018
  • zgc:77325
  • wu:fb52g04
  • wu:fj48f12
  • zgc:111900
  • MGC82178
  • DDBDRAFT_0186679
  • DDBDRAFT_0266388
  • DDB_0186679
  • DDB_0266388
  • DHS
  • DS
  • MIG13
  • Dhs
  • DYS
  • EDA22
  • K18J17.7
  • K18J17_7
  • deoxyhypusine synthase
  • embryo sac development arrest 22
  • deoxyhypusine synthase
  • Deoxyhypusine synthase
  • DHPS
  • dhps
  • Shell_1081
  • PF14_0125
  • CNJ02480
  • Tb10.70.4900
  • Tb927.1.870
  • Mmah_1888
  • Tagg_0666
  • Tagg_1037
  • Mesil_2448
  • Trad_0088
  • Igag_1842
  • Desac_0724
  • Mzhil_1083
  • Mzhil_1149
  • Mzhil_1233
  • Dhps
  • DHS
Reactivity
Human
69
24
20
5
2
2
2
2
1
1
Host
Rabbit
62
8
Clonality
Polyclonal
Conjugate
Un-conjugated
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Application
Western Blotting (WB)
55
23
13
8
7
4
4
3
Options
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Specificity This antibody detects endogenous levels of DHS protein. (region surrounding Leu87)
Cross-Reactivity (Details) Species reactivity (tested):Human.
Purification Affinity chromatography
Purity > 95 % by SDS-PAGE
Background Deoxyhypusine synthase (DHS) is crucial for the posttranslational formation of hypusine, a modification of a specific lysine residue in eukaryotic initiation factor 5A (eIF-5A). Hypusine is formed by posttranslational modifications involving two enzymatic steps catalyzed by DHS and deoxyhypusine hydroxylase (DOHH). eIF-5A is essential for eukaryotic cell proliferation. DHS, which belongs to the deoxyhypusine synthase family of proteins, is important for the first step in the hypusine biosynthesis pathway. It acts as a catalyst for the NAD-dependent oxidative cleavage of spermidine and the ensuing transfer of the butylamine moiety of spermidine to the eIF-5A protein, to create the intermediate deoxyhypusine residue.
Molecular Weight approx. 45 kDa
Gene ID 1725
NCBI Accession NP_001921
UniProt P49366
Application Notes Western Blot: 1/500 - 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1,0 mg/mL
Buffer Phosphate buffered saline (PBS), pH 7.2., 0.05 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
Western Blotting (WB) image for anti-Deoxyhypusine Synthase (DHPS) antibody (ABIN499058) Western blot (WB) analysis of DHS antibody in extracts from 3T3 cells.
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