POLR3D antibody

Catalog No. ABIN499065

Product Details anti-POLR3D Antibody

Target: POLR3D (Polymerase (RNA) III (DNA Directed) Polypeptide D, 44kDa (POLR3D))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This POLR3D antibody is un-conjugated

Application: Western Blotting (WB)

Specificity: This antibody detects endogenous levels of POLR3D protein. (region surrounding asn314)

Cross-Reactivity (Details): Species reactivity (tested):Human, Mouse.

Purification: Affinity chromatography using epitope-specific immunogen

Purity: > 95 % (by SDS-PAGE)

Application Details

Application Notes: Western blot: 1: 500 - 1: 1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.2, 0.05 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.

Target Details for POLR3D

Target: POLR3D (Polymerase (RNA) III (DNA Directed) Polypeptide D, 44kDa (POLR3D))

Alternative Name: POLR3D (POLR3D Products)

Synonyms: 2810426M17Rik antibody, 44kDa antibody, AI326118 antibody, AW489084 antibody, BN51T antibody, RPC4 antibody, TSBN51 antibody, RPC53 antibody, RNA polymerase III subunit D antibody, polymerase (RNA) III (DNA directed) polypeptide D antibody, Polr3d antibody, POLR3D antibody

Background: Three genes (POLR2 J1, POLR2 J2, and POLR2 J3) form a cluster of total length of 214 530 bp in the genetic locus 7q22.1 on the long arm of chromosome 7 (contig NT_007933). The fourth gene (POLR2 J4, 31 040 bp) was localized in the cytogenetic locus 7p13 of the short arm of chromosome 7 (contig NT_007819). An analysis enabled us to refine dissimilar experimental data on the mapping of the hRPB11 subunit gene on chromosome 7. In particular, the presence of three sites of its localization according to data on hybridization with fluorescent-labeled probes (the FISH method) was explained. It was established that, upon the expression of the four human POLR2 J genes, at least 14 types of mature mRNAs encoding somewhat differing hRPB11 isoforms can be synthesized. Eleven of these mRNAs were revealed (as full-length copies or clearly identifiable fragments) in the available databases of expressed sequence tags and cDNAs. The most probable scheme of origination of the multiple genes of the POLR2 J family as a result of three consecutive segmented duplications increasing in size was proposed and substantiated.Synonyms: BN51, BN51T, DNA-directed RNA polymerase III subunit D, DNA-directed RNA polymerase III subunit RPC4, RNA polymerase III 47 kDa subunit, RNA polymerase III subunit C4, RPC4, RPC53 homolog

Molecular Weight: approx. 38 kDa

Gene ID: 661

NCBI Accession: NP_001713

UniProt: P05423