CRISP2 antibody (AA 81-93)

Catalog No. ABIN499113

Product Details anti-CRISP2 Antibody

Target: CRISP2 (Cysteine-Rich Secretory Protein 2 (CRISP2))

Binding Specificity: AA 81-93

Reactivity: Human, Dog, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This CRISP2 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Sequence: C-QHSDPEDRKT STR

Specificity: Recognizes CRISP2.

Cross-Reactivity (Details): Species reactivity (expected):Human, Pig, Canine.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Synthetic peptide corresponding to internal sequence amino acids 81-93 of Human CRISP2 (NP_003287.1).

Application Details

Application Notes: Peptide ELISA: Detection Limit: 1/8000. Western blot: 0.5-1.0 μg/mL. In transfected HEK293 transiently expressing Human CRISP2(MYC and DYKDDDDK tagged), a band of approx. 35 kDa was observed. No bands were observed inmock-transfected HEK293 and the same band was observed using anti-myc tag antibody.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for CRISP2

Target: CRISP2 (Cysteine-Rich Secretory Protein 2 (CRISP2))

Alternative Name: CRISP2 (CRISP2 Products)

Synonyms: ct36 antibody, tpx1 antibody, tsp1 antibody, crisp antibody, gapdl5 antibody, crisp-2 antibody, CRISP3 antibody, CRISP2 antibody, CRISP-2 antibody, GAPDL5 antibody, Tpx-1 antibody, Tpx1 antibody, Crisp2l antibody, CT36 antibody, TPX1 antibody, TSP1 antibody, CRISP-3 antibody, CRISP-1 antibody, Crisp2 antibody, cysteine-rich secretory protein 2 L homeolog antibody, cysteine rich secretory protein 2 antibody, cysteine-rich secretory protein 2 antibody, crisp2.L antibody, CRISP2 antibody, Crisp2 antibody

Background: Cysteine-rich secretory protein 2 (CRISP2) may regulate some ion channels' activity and therebye regulate calcium fluxes during sperm capacitation. CRISP2 is specific to testis and epididymis.Synonyms: CRISP-2, CT36, Cancer/testis antigen 36, Cysteine-rich secretory protein 2, GAPDL5, TPX1, Testis-specific protein TPX-1

Gene ID: 7180

NCBI Accession: NP_001135879

UniProt: P16562