ACO2 antibody (Internal Region)

Catalog No. ABIN499115

Product Details anti-ACO2 Antibody

Target: ACO2 (Aconitase 2, Mitochondrial (ACO2))

Binding Specificity: AA 541-555, Internal Region

Reactivity: Human, Rat, Mouse, Pig, Cow, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This ACO2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Sequence: C-QDTYQHPPKD SSGQH

Specificity: This antibody recognizes Aconitase 2 (541-555).

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Canine (Dog), Bovine, Pig.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide

Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_001089.1. Genename: ACO2

Application Details

Application Notes: Peptide ELISA: Detection Limit: 1/6000. Western blot: 0.01-0.03 μg/mL. Approx 90 kDa band observed in Human Heart lysates(calculated MW of 85.0 kDa according to NP_001089.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH ~7.3, 0.02 % Sodium Azide, 0.5 % BSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for ACO2

Target: ACO2 (Aconitase 2, Mitochondrial (ACO2))

Alternative Name: ACO2 (ACO2 Products)

Synonyms: DDBDRAFT_0206187 antibody, DDBDRAFT_0230168 antibody, DDB_0206187 antibody, DDB_0230168 antibody, aconitase 2 antibody, F10M23.310 antibody, F10M23_310 antibody, ACONM antibody, ICRD antibody, Aco-2 antibody, Aco3 antibody, D10Wsu183e antibody, cb1017 antibody, wu:fa10e03 antibody, wu:fb69g04 antibody, wu:fc20c11 antibody, aconitase 2 antibody, aconitase 2 S homeolog antibody, aconitate hydratase, mitochondrial antibody, aconitase, mitochondrial antibody, mitochondrial aconitate hydratase antibody, aconitase 2, mitochondrial antibody, Probable aconitate hydratase, mitochondrial antibody, ACO2 antibody, aco2.S antibody, Bm1_07420 antibody, aco2 antibody, ach1 antibody, Aco2 antibody, aco-2 antibody

Background: Aconitase 2 (ACO2) is an essential iron-dependent metabolic enzyme that catalyzes the interconversion of citrate to isocitrate via cis-aconitate in the second step of the TCA cycle.Synonyms: Aconitase 2, Aconitate hydratase mitochondrial

Gene ID: 50

NCBI Accession: NP_001089

UniProt: Q99798