ETFA antibody (C-Term)

Catalog No. ABIN499133

Product Details anti-ETFA Antibody

Target: ETFA (Electron-Transfer-Flavoprotein, alpha Polypeptide (ETFA))

Binding Specificity: C-Term

Reactivity: Human, Mouse, Rat, Cow, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This ETFA antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Sequence: C-QVADYGIVAD LFK

Specificity: This antibody is expected to recognize both reported isoforms (NP_000117.1 and NP_001121188.1).

Cross-Reactivity (Details): Species reactivity (expected):Canine, Bovine.
Species reactivity (tested):Human, Mouse, Rat.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence from the C Terminus of the protein sequence according to NP_000117.1 and NP_001121188.1. Genename: ETFA

Application Details

Application Notes: Peptide ELISA: Limit Dilution: 1/8000. Western blot: 0.05-0.2 μg/mL. This antibody detects a band of approx. 30 kDa in Human, Mouseand Rat Heart lysates.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for ETFA

Target: ETFA (Electron-Transfer-Flavoprotein, alpha Polypeptide (ETFA))

Alternative Name: ETFA (ETFA Products)

Synonyms: 2010200I21Rik antibody, D9Ertd394e antibody, ETF antibody, EMA antibody, GA2 antibody, MADD antibody, cb1020 antibody, fd06h11 antibody, wu:fd06h11 antibody, ema antibody, ga2 antibody, madd antibody, electron transfer flavoprotein alpha subunit antibody, electron-transfer-flavoprotein, alpha polypeptide antibody, putative electron-transfer-flavoprotein,alpha polypeptide antibody, electron transfer flavoprotein subunit alpha, mitochondrial antibody, electron transferring flavoprotein, alpha polypeptide antibody, electron transfer flavoprotein alpha subunit L homeolog antibody, ETFA antibody, Tc00.1047053503559.109 antibody, Tc00.1047053511693.90 antibody, LMJF_28_1140 antibody, LOC100194695 antibody, etfa antibody, Etfa antibody, etfa.L antibody

Background: ETFA participates in catalyzing the initial step of the mitochondrial fatty acid beta-oxidation. It shuttles electrons between primary flavoprotein dehydrogenases and the membrane-bound electron transfer flavoprotein ubiquinone oxidoreductase. Defects in electron-transfer-flavoprotein have been implicated in type II glutaricaciduria in which multiple acyl-CoA dehydrogenase deficiencies result in large excretion of glutaric, lactic, ethylmalonic, butyric, isobutyric, 2-methyl-butyric, and isovaleric acids. Two transcript variants encoding different isoforms have been found for this gene.Synonyms: Alpha-ETF, Electron transfer flavoprotein subunit alpha

Molecular Weight: 30.0kDa (NP_001121188.1).

Gene ID: 2108

NCBI Accession: NP_000117

UniProt: P13804

Pathways: SARS-CoV-2 Protein Interactome