NDUFS7 antibody (Internal Region)

Catalog No. ABIN499134

Product Details anti-NDUFS7 Antibody

Target: NDUFS7 (NADH Dehydrogenase (Ubiquinone) Fe-S Protein 7, 20kDa (NADH-Coenzyme Q Reductase) (NDUFS7))

Binding Specificity: Internal Region

Reactivity: Human, Mouse, Rat, Cow, Dog

Host: Goat

Clonality: Polyclonal

Conjugate: This NDUFS7 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Sequence: C-SRGEYVVAKL D

Specificity: Recognizes NDUFS7.

Cross-Reactivity (Details): Species reactivity (expected):Mouse, Rat, Canine (Dog), Bovine.
Species reactivity (tested):Human.

Purification: Ammonium Sulphate Precipitation followed by Antigen Affinity Chromatography using the immunizing peptide.

Immunogen: Peptide with sequence from the internal region of the protein sequence according to NP_077718.3. Genename: NDUFS7

Application Details

Application Notes: Peptide ELISA: Limit Dilution: 1/16000. Western blot: 1-3 μg/mL. This antibody detects a band of approx. 20 kDa in Human Heart andHuman Skeletal Muscle lysates.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, pH 7.3 containing 0.02 % Sodium Azide as preservative and 0.5 % BSA as stabilizer.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for NDUFS7

Target: NDUFS7 (NADH Dehydrogenase (Ubiquinone) Fe-S Protein 7, 20kDa (NADH-Coenzyme Q Reductase) (NDUFS7))

Alternative Name: NDUFS7 (NDUFS7 Products)

Synonyms: 1010001M04Rik antibody, CI-20 antibody, CI-20KD antibody, MY017 antibody, PSST antibody, NADH dehydrogenase (ubiquinone) Fe-S protein 7 antibody, NADH:ubiquinone oxidoreductase core subunit S7 antibody, Ndufs7 antibody, NDUFS7 antibody

Background: Defects in NDUFS7 are a cause of Leigh syndrome (LS) [MIM:256000]. LS is a severe neurological disorder characterized by bilaterally symmetrical necrotic lesions in subcortical brain regions. Defects in NDUFS7 are a cause of mitochondrial complex I deficiency (MT-C1D) [MIM:252010]. A disorder of the mitochondrial respiratory chain that causes a wide range of clinical disorders, from lethal neonatal disease to adult-onset neurodegenerative disorders. Phenotypes include macrocephaly with progressive leukodystrophy, non-specific encephalopathy, cardiomyopathy, myopathy, liver disease, Leigh syndrome, Leber hereditary optic neuropathy, and some forms of Parkinson disease.Synonyms: CI-20kD, Complex I-20kD, NADH dehydrogenase [ubiquinone] iron-sulfur protein 7, NADH-ubiquinone oxidoreductase 20 kDa subunit, PSST subunit, mitochondrial

Gene ID: 374291

NCBI Accession: NP_077718

UniProt: O75251