CD90 antibody (Internal Region)

Catalog No. ABIN499172

Product Details anti-CD90 Antibody

Target: CD90 (THY1)

Binding Specificity: Internal Region

Reactivity: Human, Dog, Pig

Host: Goat

Clonality: Polyclonal

Conjugate: This CD90 antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: C-PEHTYRSRTN FTSKY

Specificity: This antibody detects CD90 (Internal).

Cross-Reactivity (Details): Species reactivity (expected):Dog, pigSpecies reactivity (tested):Human

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from the internal region of the protein sequence according to NP_006279.2

Application Details

Application Notes: Peptide ELISA: Limit dilution 1: 64000. Western blot: 0.1-0.3 μg/mL. Approx 26 kDa band observed in Human Brain (Cerebellum)and Human Tonsil lysates (calculated MW of 17.6 kDa according to NP_006279.2). Theobserved molecular weight corresponds to earlier findings with different antibodies fromother commercial sources.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C up to one week or (in aliquots) at -20 °C for longer.

Target Details for CD90

Target: CD90 (THY1)

Alternative Name: CD90 (THY1 Products)

Synonyms: CD90 antibody, CD7 antibody, T25 antibody, Thy-1 antibody, Thy-1.2 antibody, Thy1.1 antibody, Thy1.2 antibody, Thy-1 cell surface antigen antibody, thymus cell antigen 1, theta antibody, THY1 antibody, Thy1 antibody

Background: CD90 / Thy1 antigen is a GPI linked glycoprotein member of the Immunoglobulin superfamily. It is expressed on murine T cells, thymocytes, neural cells, cells of granulocytic lineage, early hematopoietic progenitors, fibroblasts, neurons and Kupffer's cells. Thy1 may play a role in cell to cell or cell to ligand interactions during synaptogenesis and other events in the brain. It is found in most mouse strains except AKR/J, A, Thy1.1 and B6.PL (74NS) expressing Thy1.1.Synonyms: CDw90, THY1, Thy-1, Thy-1 membrane glycoprotein

Gene ID: 7070

NCBI Accession: NP_006279

UniProt: P04216

Pathways: Cell-Cell Junction Organization