Serotonin Receptor 3B antibody (Internal Region)

Catalog No. ABIN499176

Product Details anti-Serotonin Receptor 3B Antibody

Target: Serotonin Receptor 3B (HTR3B)

Binding Specificity: Internal Region

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This Serotonin Receptor 3B antibody is un-conjugated

Application: Western Blotting (WB)

Sequence: C-SNYLQTQDQT DQQE

Specificity: This antibody detects Serotonin receptor 3B (Internal).

Cross-Reactivity (Details): Species reactivity (tested):Human

Purification: Ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide

Immunogen: Peptide from the internal region (near C-terminus) of the protein sequence according to NP_006019.1

Application Details

Application Notes: Peptide ELISA: 1: 16000 (limit dilution). Western blot: 1-3 μg/mL. Approx 51 kDa band observed in Human Brain (Cerebral Cortex,Hippocampus and Amygdala) lysates (calculated MW of 50.1 kDa according toNP_006019.1).
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0.5 mg/mL

Buffer: Tris saline, 0.02 % sodium azide, pH 7.3 with 0.5 % bovine serum albumin

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store at 2 - 8 °C up to one week or (in aliquots) at -20 °C for longer.

Target Details for Serotonin Receptor 3B

Target: Serotonin Receptor 3B (HTR3B)

Abstract: HTR3B Products

Synonyms: HTR3B antibody, 5-HT3B antibody, 5-hydroxytryptamine receptor 3B antibody, 5-hydroxytryptamine (serotonin) receptor 3B antibody, HTR3B antibody, Htr3b antibody

Background: There are 4 main groups of receptors which bind the neurotransmitter serotonin: 5HT1, 5HT2 5HT3 and 5HT4. 5HT3B receptor is subunit B of the type 3 receptor for 5-hydroxytryptamine (serotonin). This receptor causes fast, depolarizing responses in neurons after activation. It appears that the heteromeric combination of A and B subunits is necessary to provide the full functional features of this receptor, since either subunit alone results in receptors with very low conductance and response amplitude. Through interactions with these receptors, serotonin is able to exert a wide variety of physiological functions. 5HT3 receptors are cation-selective transmitter-gated ion channels. 5HT3B receptor mutations have been implicated in depression.Synonyms: 5-HT3-B, 5-HT3B, 5-hydroxytryptamine receptor 3B

Gene ID: 9177

NCBI Accession: NP_006019

UniProt: O95264

Pathways: Synaptic Membrane