Search, Find and Buy Antibodies, ELISA Kits and Proteins.
Loading Loading
Order hotline:
phone +1 404 474 4654
fax +1 888 205 9894 (TF)
Details for Product No. ABIN499225

Amino-terminal Enhancer of Split (AES) (N-Term), (C-Term) antibody

Request
Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Synonyms GRG, ESP1, GRG5, TLE5, AES-1, AES-2, AL024115, Esp1, Grg, Grg-5, Grg5, Tle5, zgc:64216, aes-1, aes-2, esp1, grg, grg5, tle5, xgrg-5, AES
Epitope
»Alternatives N-Term, C-Term
Reactivity
»Alternatives Human, Mouse (Murine), Rat (Rattus)
Host
»Alternatives Rabbit
Clonality Polyclonal
Application
»Alternatives ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
Pubmed 4 references available
Catalog no. ABIN499225
Quantity 0.1 mg
Price
390.50 $   Plus shipping costs $45.00
Shipping to
Availability Will be delivered in 6 to 8 Business Days
Immunogen Human AES / Amino-terminal enhancer of split / GRG / ESP1 / TLE5 (C-Terminus) Peptide (AP30024CP-N)
Isotype IgG
Specificity AES antibody was raised against a 16 amino acid peptide from near the carboxy terminus of human AES.
Purification Affinity chromatography purified via peptide column
Alternative Name AES / ESP1
Background Adhesion to extracellular matrix regulates cell survival through both integrin engagement and appropriate cell spreading. Anoikis is the molecular mechanism of apop-tosis induced by integrin detachment (1). Amino-terminal enhancer of split (AES) is a member of the Groucho/ transducin-like enhancer of split (TLE) family of transcriptional regulators, a group of transcriptional co-repressors that play important roles in neurogenesis, segmentation, and sex determination (2,3). AES forms a complex with Bit1 (Bcl-2 inhibitor of transcription 1), a mitochondrial protein that is released into the cytoplasm upon onset of apoptosis (4). It has been suggested that this complex turns off a survival-promoting gene transcription program controlled by the TLE protein family. (4). Interestingly, apoptosis of cells transfected with AES and Bit1 could be inhibited if the cells were allowed to attach to fibronectin through the alpha5beta1 integrin suggesting that the Bit1-AES pathway contributing to anoikis is regulated by integrins, and in particular, the alpha5beta1 integrin (4).
Alternate names: AES1, Amino-terminal enhancer of split, GRG protein, Gp130-associated protein GAM
Gene ID 166
NCBI Accession NP_001121
UniProt Q08117
Application Notes ELISA. Western Blot: AES antibody can be used for the detection of AES at 1 - 2 μg/ml. Immunocytochemistry. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Buffer PBS containing 0.02% Sodium Azide as preservative
Preservative Sodium azide
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Expiry Date 12 months
Background publications Miyasaka, Choudhury, Hou et al.: "Molecular cloning and expression of mouse and human cDNA encoding AES and ESG proteins with strong similarity to Drosophila enhancer of split groucho protein." in: European journal of biochemistry / FEBS, Vol. 216, Issue 1, pp. 343-52, 1993 (PubMed).

Chen, Courey: "Groucho/TLE family proteins and transcriptional repression." in: Gene, Vol. 249, Issue 1-2, pp. 1-16, 2000 (PubMed).

Jan, Matter, Pai et al.: "A mitochondrial protein, Bit1, mediates apoptosis regulated by integrins and Groucho/TLE corepressors." in: Cell, Vol. 116, Issue 5, pp. 751-62, 2004 (PubMed).

Martin, Vuori: "Regulation of Bcl-2 proteins during anoikis and amorphosis." in: Biochimica et biophysica acta, Vol. 1692, Issue 2-3, pp. 145-57, 2004 (PubMed).

Alternatives for antigen "Amino-terminal Enhancer of Split (AES)", type "Antibodies"
Hosts (35), (5), (1)
Reactivities (38), (24), (14), (2), (1), (1), (1)
Applications (36), (18), (18), (10), (5), (2), (2), (1), (1)
Epitopes (13), (9), (3), (2), (2), (1), (1)
Beacon