Amino-terminal Enhancer of Split (AES) (N-Term) antibody

Details for Product No. ABIN499227
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Antigen
Synonyms grg, esp1, grg5, tle5, aes-1, aes-2, AES, AES-1, AES-2, ESP1, GRG, GRG5, TLE5, AL024115, Esp1, Grg, Grg-5, Grg5, Tle5, zgc:64216, xgrg-5
Epitope
N-Term
(30), (17), (7), (2), (2), (2), (2), (1), (1), (1)
Reactivity
Human, Mouse (Murine)
(60), (31), (18), (3), (2), (2), (1), (1)
Host
Rabbit
(55), (5), (2)
Clonality
Polyclonal
Conjugate
Un-conjugated
(1), (1), (1), (1), (1), (1)
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
(58), (27), (18), (7), (5), (2), (2), (2), (1)
Pubmed 4 references available
Quantity 0.1 mg
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Catalog No. ABIN499227
401.50 $
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Immunogen Human AES / Amino-terminal enhancer of split / GRG / ESP1 / TLE5 (N-Terminus) Peptide
Isotype IgG
Specificity AES antibody was raised against a 16 amino acid peptide from near the amino-terminus of human AES.
Purification Affinity chromatography purified via peptide column
Alternative Name AES / ESP1
Background Adhesion to extracellular matrix regulates cell survival through both integrin engagement and appropriate cell spreading. Anoikis is the molecular mechanism of apop-tosis induced by integrin detachment (1). Amino-terminal enhancer of split (AES) is a member of the Groucho/ transducin-like enhancer of split (TLE) family of transcriptional regulators, a group of transcriptional co-repressors that play important roles in neurogenesis, segmentation, and sex determination (2,3). AES forms a complex with Bit1 (Bcl-2 inhibitor of transcription 1), a mitochondrial protein that is released into the cytoplasm upon onset of apoptosis (4). It has been suggested that this complex turns off a survival-promoting gene transcription program controlled by the TLE protein family. (4). Interestingly, apoptosis of cells transfected with AES and Bit1 could be inhibited if the cells were allowed to attach to fibronectin through the alpha5beta1 integrin suggesting that the Bit1-AES pathway contributing to anoikis is regulated by integrins, and in particular, the alpha5beta1 integrin (4).Synonyms: AES1, Amino-terminal enhancer of split, GRG protein, Gp130-associated protein GAM
Gene ID 166
NCBI Accession NP_001121
UniProt Q08117
Application Notes ELISA. Western Blot: AES antibody can be used for the detection of AES at 2 - 4 μg/mL. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Buffer PBS containing 0.02 % Sodium Azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Background publications Miyasaka, Choudhury, Hou et al.: "Molecular cloning and expression of mouse and human cDNA encoding AES and ESG proteins with strong similarity to Drosophila enhancer of split groucho protein." in: European journal of biochemistry / FEBS, Vol. 216, Issue 1, pp. 343-52, 1993 (PubMed).

Chen, Courey: "Groucho/TLE family proteins and transcriptional repression." in: Gene, Vol. 249, Issue 1-2, pp. 1-16, 2000 (PubMed).

Jan, Matter, Pai et al.: "A mitochondrial protein, Bit1, mediates apoptosis regulated by integrins and Groucho/TLE corepressors." in: Cell, Vol. 116, Issue 5, pp. 751-62, 2004 (PubMed).

Martin, Vuori: "Regulation of Bcl-2 proteins during anoikis and amorphosis." in: Biochimica et biophysica acta, Vol. 1692, Issue 2-3, pp. 145-57, 2004 (PubMed).

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