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APAF1 antibody (Apoptotic Peptidase Activating Factor 1) (N-Term)

Details for Product anti-APAF1 Antibody No. ABIN499287, Supplier: Log in to see
Antigen
  • 6230400I06Rik
  • anon-53Fa
  • apaf-1
  • Apaf-1
  • APAF-1
  • Apaf1
  • apaf1
  • APAF1
  • Apaf1l
  • arc
  • ARK
  • ark
  • CED4
  • CG6829
  • D-Apaf-1
  • dapaf
  • Dapaf-1
  • dAPAF-1
  • dapaf-1
  • dApaf-1
  • dApaf-1/DARK/HAC-1
  • Dapaf-1/HAC-1
  • dapaf-1L
  • dapaf-1S
  • dApaf1
  • DARK
  • dark
  • dArk
  • Dark
  • Dark/Apaf-I
  • dark/dapaf-1/hac-1
  • Dark/Dapaf-1/HAC1
  • Dark/Hac-1/dApaf-1
  • dark/hac-1/dapaf-1
  • Dark/Hac-1/dApaf1
  • Dmel\\CG6829
  • fog
  • hac-1
  • Hac-1
  • Hac-1/Dark
  • hac1
  • Hac1
  • l(2)SH0173
  • mKIAA0413
  • T1
  • zgc:136543
Alternatives
anti-Human APAF1 antibody for Western Blotting
Epitope
N-Term
46
43
17
13
13
8
5
4
4
3
3
3
2
2
2
2
2
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
201
110
90
4
2
2
2
1
1
1
Host
Rabbit
172
25
13
4
Clonality
Polyclonal
Conjugate
This APAF1 antibody is un-conjugated
3
3
2
2
2
2
2
2
2
2
2
2
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
158
71
64
51
34
22
21
13
9
4
4
Supplier
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Immunogen Human Apaf1 (N-Terminus) Peptide
Isotype IgG
Specificity Apaf1 antibody was raised against a peptide corresponding to amino acids near the amino terminus of human Apaf1. The sequences of the immunogenic peptide are identical between human and mouse.
Purification Affinity chromatography purified via peptide column
Alternative Name APAF1 (APAF1 Antibody Abstract)
Background Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. The mammalian homologues of the key cell death gene CED-4 in C. elegans has been identified recently from human and mouse and designated Apaf1 (for apoptosis protease-activating factor 1) (1,2). Apaf1 binds to cytochrome c (Apaf-2) and caspase-9 (Apaf-3), which leads to caspase-9 activation. Activated caspase-9 in turn cleaves and activates caspase-3 that is one of the key proteases, being responsible for the proteolytic cleavage of many key proteins in apoptosis (3). Apaf1 can also associate with caspase-4 and caspase-8 (4). Apaf1 is ubiquitously expressed in human tissues .Synonyms: Apaf-1, Apoptotic protease-activating factor 1, KIAA0413
Gene ID 317
UniProt O14727
Research Area Cancer, Apoptosis/Necrosis
Pathways p53 Signaling, Apoptosis, Caspase Cascade in Apoptosis
Application Notes ELISA. Western Blot: Apaf1 antibody can be used for detection of Apaf1 at 1 - 2 μg/mL. Whole celllysate from HeLa cells can be used as positive control and a 115 - 130 kDa band should bedetected. Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Buffer PBS containing 0.02 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8 °C.
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-APAF1 antibody (Apoptotic Peptidase Activating Factor 1) (N-Term) (ABIN499287) Immunohistochemistry of Apaf1 in human heart tissue with Apaf1 antibody at 1 μg/ml.
Western Blotting (WB) image for anti-APAF1 antibody (Apoptotic Peptidase Activating Factor 1) (N-Term) (ABIN499287) Western blot analysis of Apaf1 in K562 cell lysate with AP30055PU-N Apaf1 antibody at...
Background publications Hu, Benedict, Wu, Inohara, Núñez: "Bcl-XL interacts with Apaf-1 and inhibits Apaf-1-dependent caspase-9 activation." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, Issue 8, pp. 4386-91, 1998 (PubMed).

Cecconi, Alvarez-Bolado, Meyer, Roth, Gruss: "Apaf1 (CED-4 homolog) regulates programmed cell death in mammalian development." in: Cell, Vol. 94, Issue 6, pp. 727-37, 1998 (PubMed).

Zou, Henzel, Liu, Lutschg, Wang: "Apaf-1, a human protein homologous to C. elegans CED-4, participates in cytochrome c-dependent activation of caspase-3." in: Cell, Vol. 90, Issue 3, pp. 405-13, 1997 (PubMed).

Li, Nijhawan, Budihardjo, Srinivasula, Ahmad, Alnemri, Wang: "Cytochrome c and dATP-dependent formation of Apaf-1/caspase-9 complex initiates an apoptotic protease cascade." in: Cell, Vol. 91, Issue 4, pp. 479-89, 1997 (PubMed).