You are viewing an incomplete version of our website. Please click to reload the website as full version.

ANAPC5 antibody (Anaphase Promoting Complex Subunit 5) (Center)

Details for Product anti-ANAPC5 Antibody No. ABIN499299, Supplier: Login to see
Antigen
  • DDBDRAFT_0206386
  • DDBDRAFT_0234269
  • DDB_0206386
  • DDB_0234269
  • zgc:55800
  • wu:fb75a09
  • ANAPC5
  • MGC89697
  • APC5
  • 2510006G12Rik
  • AA408751
  • AA536819
  • AA986414
  • Anpc5
  • F12K11.7
  • F12K11_7
Epitope
Center
18
17
12
3
2
2
2
2
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
90
32
30
Host
Rabbit
89
1
Clonality
Polyclonal
Conjugate
This ANAPC5 antibody is un-conjugated
6
6
6
3
2
2
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
69
47
30
15
11
7
5
3
2
1
Supplier
Login to see
Supplier Product No.
Login to see
Request

Showcase your results, aid the scientific community, and receive a full refund.

Contribute a validation

Learn more

Available images

Immunogen APC5 antibody was raised against a 17 amino acid peptide near the center of human APC5.
Isotype IgG
Purification Affinity chromatography purified via peptide column
Alternative Name APC5 / ANAPC5 (ANAPC5 Antibody Abstract)
Background Cell cycle regulated protein ubiquitination and degradation within subcellular domains is thought to be essential for the normal progression of mitosis. APC5 is a highly conserved component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC/C is responsible for degrading anaphase inhibitors, mitotic cyclins, and spindle-associated proteins ensuring that events of mitosis take place in proper sequence. The individual APC/C components mRNA and protein levels are expressed at approximately the same levels in most tissues and cell lines, suggesting that they perform their functions as part of a complex. While little is known of APC5, it is thought that APC5 associates with other APC/C components APC1, APC4, and CDC23 interdependently, such that loss of any one subunit reduces binding between the remaining three.Synonyms: Anaphase-promoting complex subunit 5, Cyclosome subunit 5
Gene ID 51433
UniProt Q9UJX4
Research Area Coagulation
Pathways
Application Notes ELISA. Western Blot: APC5 antibody can be used for detection of APC5 at 1 - 2 μg/mL.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS containing 0.02 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody (in aliquots) at -20 °C.
Supplier Images
Western Blotting (WB) image for anti-ANAPC5 antibody (Anaphase Promoting Complex Subunit 5) (Center) (ABIN499299) Western blot analysis of APC5 in human kidney tissue lysate with AP30061PU-N APC5 ant...
Background publications Peters: "The anaphase promoting complex/cyclosome: a machine designed to destroy." in: Nature reviews. Molecular cell biology, Vol. 7, Issue 9, pp. 644-56, 2006 (PubMed).

Thornton, Ng, Matyskiela et al.: "An architectural map of the anaphase-promoting complex." in: Genes & development, Vol. 20, Issue 4, pp. 449-60, 2006 (PubMed).

Jörgensen, Gräslund, Betz et al.: "Characterisation of the human APC1, the largest subunit of the anaphase-promoting complex." in: Gene, Vol. 262, Issue 1-2, pp. 51-9, 2001 (PubMed).