You are viewing an incomplete version of our website. Please click to reload the website as full version.

ATF6 antibody (Activating Transcription Factor 6) (C-Term)

Details for Product anti-ATF6 Antibody No. ABIN499351, Supplier: Log in to see
Antigen
  • ATF-6-like
  • GB16435
  • ATF6
  • Atf6
  • ATF6A
  • 9130025P16Rik
  • 9630036G24
  • AA789574
  • Atf6alpha
  • ESTM49
Epitope
C-Term
22
21
14
9
5
5
4
3
3
2
2
2
2
2
2
2
2
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
126
38
16
1
1
1
1
1
Host
Rabbit
92
22
15
Clonality
Polyclonal
Conjugate
This ATF6 antibody is un-conjugated
4
3
3
3
3
3
1
1
1
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
105
62
27
27
21
12
12
6
5
5
3
2
2
1
Supplier
Log in to see
Supplier Product No.
Log in to see
Request

Showcase your results, aid the scientific community, and receive a full refund.

Contribute a validation

Learn more

Available images

Immunogen Human ATF6 (C-Terminus) Peptide
Isotype IgG
Specificity This antibody was raised against a 16 amino acid peptide from near the carboxy terminus of Human ATF6. ATF6 has two isoforms (ATF6? and ATF6B), only ATF6? is recognized by this antibody.
Purification Affinity chromatography purified via peptide column
Alternative Name ATF6 alpha (ATF6 Antibody Abstract)
Background Disruptions of protein folding and maturation in the endoplasmic reticulum (ER) result in the activation of the unfolded protein response (UPR), an integrated cellular signaling pathway that transmits information from the ER lumen to the cytoplasm and nucleus. Activating transcription factor 6 (ATF6) as well as the ER-transmembrane protein kinases IRE1p and PERK are the major transducers of the UPR . ATF6 is an ER transmembrane protein that is normally bound to the ER chaperone GRP78, but upon ER stress is released from GRP78 and proteolytically cleaved to yield a cytosolic fragment which then migrates to the nucleus, and together with the transcription factor XBP-1, activates transcription of UPR-responsive genes (2-4).Synonyms: ATF-6, Activating transcription factor 6 alpha, Cyclic AMP-dependent transcription factor ATF-6 alpha, cAMP-dependent transcription factor ATF-6 alpha
Gene ID 22926
NCBI Accession NP_031374
UniProt P18850
Application Notes ELISA. Western Blot: ATF6 antibody can be used for the detection of ATF6 at 0.5 - 1 μg/mL. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Buffer PBS containing 0.02 % Sodium Azide as preservative
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
Immunofluorescence (IF) image for anti-ATF6 antibody (Activating Transcription Factor 6) (C-Term) (ABIN499351) Immunocytochemical staining of MCF7 cells using AP30088PU-N ATF6 antibody at 10 μg/ml.
Immunofluorescence (IF) image for anti-ATF6 antibody (Activating Transcription Factor 6) (C-Term) (ABIN499351) Immunofluorescence of ATF6 in MCF7 cells with this product atF6 antibody at 10 µg/ml.
Western Blotting (WB) image for anti-ATF6 antibody (Activating Transcription Factor 6) (C-Term) (ABIN499351) Western blot analysis of ATF6 in MCF7 cell lysate with this product atF6 antibody at ...
Background publications Liu, Kaufman: "The unfolded protein response." in: Journal of cell science, Vol. 116, Issue Pt 10, pp. 1861-2, 2003 (PubMed).

Yoshida, Matsui, Yamamoto et al.: "XBP1 mRNA is induced by ATF6 and spliced by IRE1 in response to ER stress to produce a highly active transcription factor." in: Cell, Vol. 107, Issue 7, pp. 881-91, 2002 (PubMed).

Haze, Yoshida, Yanagi et al.: "Mammalian transcription factor ATF6 is synthesized as a transmembrane protein and activated by proteolysis in response to endoplasmic reticulum stress." in: Molecular biology of the cell, Vol. 10, Issue 11, pp. 3787-99, 1999 (PubMed).

Little, Ramakrishnan, Roy et al.: "The glucose-regulated proteins (GRP78 and GRP94): functions, gene regulation, and applications." in: Critical reviews in eukaryotic gene expression, Vol. 4, Issue 1, pp. 1-18, 1995 (PubMed).