ATF6 antibody (C-Term)

Catalog No. ABIN499351

Product Details anti-ATF6 Antibody

Target: ATF6 (Activating Transcription Factor 6 (ATF6))

Binding Specificity: C-Term

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ATF6 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Specificity: This antibody was raised against a 16 amino acid peptide from near the carboxy terminus of Human ATF6. ATF6 has two isoforms (ATF6? and ATF6B), only ATF6? is recognized by this antibody.

Purification: Affinity chromatography purified via peptide column

Immunogen: Human ATF6 (C-Terminus) Peptide

Isotype: IgG

Application Details

Application Notes: ELISA. Western Blot: ATF6 antibody can be used for the detection of ATF6 at 0.5 - 1 μg/mL. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % Sodium Azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for ATF6

Target: ATF6 (Activating Transcription Factor 6 (ATF6))

Alternative Name: ATF6 alpha (ATF6 Products)

Synonyms: ATF-6-like antibody, GB16435 antibody, ATF6 antibody, Atf6 antibody, ATF6A antibody, 9130025P16Rik antibody, 9630036G24 antibody, AA789574 antibody, Atf6alpha antibody, ESTM49 antibody, activating transcription factor 6 antibody, uncharacterized LOC412432 antibody, activating transcription factor 6 S homeolog antibody, cyclic AMP-dependent transcription factor ATF-6 alpha antibody, ATF6 antibody, LOC412432 antibody, atf6.S antibody, LOC100122162 antibody, Atf6 antibody

Background: Disruptions of protein folding and maturation in the endoplasmic reticulum (ER) result in the activation of the unfolded protein response (UPR), an integrated cellular signaling pathway that transmits information from the ER lumen to the cytoplasm and nucleus. Activating transcription factor 6 (ATF6) as well as the ER-transmembrane protein kinases IRE1p and PERK are the major transducers of the UPR . ATF6 is an ER transmembrane protein that is normally bound to the ER chaperone GRP78, but upon ER stress is released from GRP78 and proteolytically cleaved to yield a cytosolic fragment which then migrates to the nucleus, and together with the transcription factor XBP-1, activates transcription of UPR-responsive genes (2-4).Synonyms: ATF-6, Activating transcription factor 6 alpha, Cyclic AMP-dependent transcription factor ATF-6 alpha, cAMP-dependent transcription factor ATF-6 alpha

Gene ID: 22926

NCBI Accession: NP_031374

UniProt: P18850

Pathways: ER-Nucleus Signaling, Unfolded Protein Response