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beta-Site APP-Cleaving Enzyme 1 (BACE) (C-Term) antibody

Details for Product No. ABIN499393, Supplier: Log in to see
Antigen
  • BACE1
  • MGC145931
  • bace2
  • MGC68881
  • MGC68482
  • BACE2
  • C76936
  • Bace
  • ASP2
  • BACE
  • HSPC104
  • zgc:77409
Epitope
C-Term
30
25
23
13
10
7
5
5
5
4
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
188
85
58
6
3
2
2
2
1
1
1
1
1
1
1
1
Host
Rabbit
183
14
6
Clonality
Polyclonal
Conjugate
Un-conjugated
7
6
3
3
2
2
2
2
2
2
2
2
2
2
2
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
126
82
48
40
22
15
10
9
8
4
3
2
2
2
2
Supplier
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Immunogen Human BACE (C-Terminus) Peptide
Isotype IgG
Specificity BACE antibody was raised against a peptide corresponding to 17 amino acids at the carboxy terminus of human BACE.
Purification Ion exchange chromatography
Alternative Name BACE1 (BACE Antibody Abstract)
Background Accumulation of the amyloid-b (Ab) plaque in the cerebral cortex is a critical event in the pathogenesis of Alzheimer's disease. Ab peptide is generated by proteolytic cleavage of the b-amyloid protein precursor (APP) at b- and g-sites by two proteases. APP is first cleaved by b-secretase, producing a soluble derivative of the protein and a membrane anchored 99-amino acid carboxy-terminal fragment (C99). The C99 fragment serves as substrate for g-secretase to generate the 4 kDa amyloid-b peptide, which is deposited in the brains of all suffers of Alzheimer's disease. The long-sought b-secretase was recently identified by several groups independently and designated β-site APP cleaving enzyme (BACE) and aspartyl protease 2 (Asp2) (1-4). BACE/Asp2 is a novel transmembrane aspartic protease and colocalizes with APP.Synonyms: ASP2, Aspartyl protease 2, BACE, BACE-1, Beta-secretase 1, Beta-site APP cleaving enzyme 1, Beta-site amyloid precursor protein cleaving enzyme 1, KIAA1149, Memapsin-2, Membrane-associated aspartic protease 2
Gene ID 23621
UniProt P56817
Research Area Proteolysis / Ubiquitin, Alzheimer's Disease, Enzymes
Application Notes ELISA. Western Blot: BACE can be used for detection of BACE at 0.5 to 1 μg/mL. An approximately70 kDa band should be detected. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Buffer PBS containing 0.02 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8 °C.
Supplier Images
Immunofluorescence (IF) image for anti-beta-Site APP-Cleaving Enzyme 1 (BACE) (C-Term) antibody (ABIN499393) Immunocytochemistry of BACE in 3T3 cells with AP30111PU-N BACE antibody at 10 μg/ml.
Western Blotting (WB) image for anti-beta-Site APP-Cleaving Enzyme 1 (BACE) (C-Term) antibody (ABIN499393) Western blot analysis of BACE in human brain tissue lysate in the absence (A) or pres...
Background publications Hussain, Powell, Howlett et al.: "Identification of a novel aspartic protease (Asp 2) as beta-secretase." in: Molecular and cellular neurosciences, Vol. 14, Issue 6, pp. 419-27, 2000 (PubMed).

Sinha, Anderson, Barbour et al.: "Purification and cloning of amyloid precursor protein beta-secretase from human brain. ..." in: Nature, Vol. 402, Issue 6761, pp. 537-40, 1999 (PubMed).

Yan, Bienkowski, Shuck et al.: "Membrane-anchored aspartyl protease with Alzheimer's disease beta-secretase activity." in: Nature, Vol. 402, Issue 6761, pp. 533-7, 1999 (PubMed).

Vassar, Bennett, Babu-Khan et al.: "Beta-secretase cleavage of Alzheimer's amyloid precursor protein by the transmembrane aspartic protease BACE." in: Science (New York, N.Y.), Vol. 286, Issue 5440, pp. 735-41, 1999 (PubMed).