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beta Actin antibody (Actin, beta) (C-Term)

Details for Product anti-ACTB Antibody No. ABIN499441, Supplier: Log in to see
Antigen
  • beta-actin
  • ps1tp5bp1
  • LOC563571
  • ACTB
  • A26C1A
  • A26C1B
  • act-2
  • actb
  • B-actin
  • LOC100136013
  • LOC100174883
  • LOC100304816
  • A
  • ACT5C
  • Ac5C
  • Act
  • Act-5C
  • Act5
  • Act5c
  • Actin
  • Actin/BAP47
  • Actin5C
  • BAP47
  • Bap47
  • CG4027
  • Dmel\\CG4027
  • M32055
  • T11
  • act
  • act 5C
  • act5C
  • actin
  • actin5C
  • anon-EST:fe2D2
  • beta-actin/Bap47
  • cyt5C
  • l(1)G0009
  • l(1)G0010
  • l(1)G0025
  • l(1)G0079
  • l(1)G0117
  • l(1)G0177
  • l(1)G0245
  • l(1)G0330
  • l(1)G0420
  • l(1)G0486
  • Bact
  • BRWS1
  • PS1TP5BP1
  • ACT-5
  • Actx
  • E430023M04Rik
  • ACTA1
  • Beta-actin
  • B-ACTZF
  • actba
  • bact
  • bactin1
  • bactzf
  • wu:fd18f05
  • POTE2alpha
  • POTEACTIN
Epitope
C-Term
136
38
16
15
10
7
7
5
4
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
483
208
180
51
49
41
39
32
29
22
18
13
11
10
9
8
7
5
4
4
4
4
3
2
2
2
1
1
1
1
1
1
1
1
1
Host
Rabbit
316
199
18
1
1
Clonality
Polyclonal
Conjugate
This beta Actin antibody is un-conjugated
22
17
10
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
466
213
145
89
82
46
38
30
12
12
11
9
8
3
2
2
2
2
1
1
1
Supplier
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Immunogen Human b-actin / Beta actin (C-Terminus) Peptide
Isotype IgG
Specificity Beta-actin antibody was raised against a 16 amino acid peptide from near the carboxy-terminus of human beta-actin.
Purification Affinity chromatography purified via peptide column
Alternative Name Actin beta / ACTB (ACTB Antibody Abstract)
Background Actins are highly conserved proteins that are involved in cell motility, structure and integrity, processes that are crucial for tissue development and the development of organism (reviewed in 1). The actin cytoskeleton is one of the principal drivers of cell motility and is capable of responding to complex signaling cascades. Recent evidence suggests that it may play key roles in regulating apoptosis and aging (2). beta actin is one of six different actin isoforms which have been identified. Like GAPDH, Β-actin is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.Synonyms: Actin cytoplasmic 1, Beta-Actin
Gene ID 60
UniProt P60709
Application Notes ELISA. Western Blot: beta-actin antibody can be used for the detection of beta-actin at 1 - 2 μg/mL. Immnocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer PBS containing 0.02 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8 °C.
Supplier Images
Immunofluorescence (IF) image for anti-beta Actin antibody (Actin, beta) (C-Term) (ABIN499441) Immunocytochemistry of beta-actin in HeLa cells with AP30136PU-N beta-actin antibody ...
Western Blotting (WB) image for anti-beta Actin antibody (Actin, beta) (C-Term) (ABIN499441) Western blot analysis of beta-actin in HeLa cell lysate with AP30136PU-N beta-actin a...
Background publications Gourlay, Ayscough: "The actin cytoskeleton: a key regulator of apoptosis and ageing?" in: Nature reviews. Molecular cell biology, Vol. 6, Issue 7, pp. 583-9, 2005 (PubMed).

Lambrechts, Van Troys, Ampe: "The actin cytoskeleton in normal and pathological cell motility." in: The international journal of biochemistry & cell biology, Vol. 36, Issue 10, pp. 1890-909, 2004 (PubMed).