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beta Actin antibody (Actin, beta) (C-Term)

Details for Product anti-ACTB Antibody No. ABIN499441, Supplier: Log in to see
Antigen
  • A
  • A26C1A
  • A26C1B
  • Ac5C
  • Act
  • act
  • act-2
  • ACT-5
  • Act-5C
  • Act5
  • act 5C
  • ACT5C
  • Act5c
  • act5C
  • ACTA1
  • ACTB
  • actb
  • actba
  • actin
  • Actin
  • Actin/BAP47
  • actin5C
  • Actin5C
  • Actx
  • anon-EST:fe2D2
  • B-actin
  • B-ACTZF
  • Bact
  • bact
  • bactin1
  • bactzf
  • BAP47
  • Bap47
  • beta-actin
  • Beta-actin
  • beta-actin/Bap47
  • BRWS1
  • CG4027
  • cyt5C
  • Dmel\\CG4027
  • E430023M04Rik
  • l(1)G0009
  • l(1)G0010
  • l(1)G0025
  • l(1)G0079
  • l(1)G0117
  • l(1)G0177
  • l(1)G0245
  • l(1)G0330
  • l(1)G0420
  • l(1)G0486
  • LOC563571
  • LOC100136013
  • LOC100174883
  • LOC100304816
  • M32055
  • POTE2alpha
  • POTEACTIN
  • ps1tp5bp1
  • PS1TP5BP1
  • T11
  • wu:fd18f05
Epitope
C-Term
162
34
16
15
10
9
7
5
4
3
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
517
245
205
68
60
56
43
38
34
28
26
22
22
17
15
15
15
13
10
10
10
9
7
5
4
4
4
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Host
Rabbit
338
208
18
1
1
Clonality
Polyclonal
Conjugate
This beta Actin antibody is un-conjugated
31
24
17
5
5
4
4
4
4
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
498
222
160
102
94
61
49
41
15
13
12
11
10
8
6
6
3
2
2
2
2
1
1
1
1
Supplier
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Immunogen Human b-actin / Beta actin (C-Terminus) Peptide
Isotype IgG
Specificity Beta-actin antibody was raised against a 16 amino acid peptide from near the carboxy-terminus of human beta-actin.
Purification Affinity chromatography purified via peptide column
Alternative Name Actin beta / ACTB (ACTB Antibody Abstract)
Background Actins are highly conserved proteins that are involved in cell motility, structure and integrity, processes that are crucial for tissue development and the development of organism (reviewed in 1). The actin cytoskeleton is one of the principal drivers of cell motility and is capable of responding to complex signaling cascades. Recent evidence suggests that it may play key roles in regulating apoptosis and aging (2). beta actin is one of six different actin isoforms which have been identified. Like GAPDH, Β-actin is constitutively expressed at high levels in almost all tissues and cell lines making it ideal for use as a loading control marker in immunoblots.Synonyms: Actin cytoplasmic 1, Beta-Actin
Gene ID 60
UniProt P60709
Application Notes ELISA. Western Blot: beta-actin antibody can be used for the detection of beta-actin at 1 - 2 μg/mL. Immnocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1 mg/mL
Buffer PBS containing 0.02 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8 °C.
Supplier Images
Immunofluorescence (IF) image for anti-beta Actin antibody (Actin, beta) (C-Term) (ABIN499441) Immunocytochemistry of beta-actin in HeLa cells with AP30136PU-N beta-actin antibody ...
Western Blotting (WB) image for anti-beta Actin antibody (Actin, beta) (C-Term) (ABIN499441) Western blot analysis of beta-actin in HeLa cell lysate with AP30136PU-N beta-actin a...
Background publications Gourlay, Ayscough: "The actin cytoskeleton: a key regulator of apoptosis and ageing?" in: Nature reviews. Molecular cell biology, Vol. 6, Issue 7, pp. 583-9, 2005 (PubMed).

Lambrechts, Van Troys, Ampe: "The actin cytoskeleton in normal and pathological cell motility." in: The international journal of biochemistry & cell biology, Vol. 36, Issue 10, pp. 1890-909, 2004 (PubMed).