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PTRH2 antibody (Middle Region)

PTRH2 Reactivity: Human, Mouse WB, IHC (p), EIA Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN499473
  • Target See all PTRH2 Antibodies
    PTRH2 (Peptidyl-tRNA Hydrolase 2 (PTRH2))
    Binding Specificity
    • 13
    • 11
    • 11
    • 6
    • 6
    • 6
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Middle Region
    Reactivity
    • 60
    • 14
    • 14
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    Human, Mouse
    Host
    • 59
    • 1
    Rabbit
    Clonality
    • 60
    Polyclonal
    Conjugate
    • 22
    • 6
    • 5
    • 5
    • 5
    • 4
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    This PTRH2 antibody is un-conjugated
    Application
    • 41
    • 27
    • 19
    • 11
    • 5
    • 4
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
    Specificity
    Bit1 antibody was raised against a 16 amino acid peptide from near the center of human Bit1.
    Purification
    Affinity chromatography purified via peptide column
    Immunogen
    Human Bit1 / Bcl-2 inhibitor of transcription 1 / Peptidyl-tRNA hydrolase 2 (Intermediate Domain) Peptide
    Isotype
    IgG
    Top Product
    Discover our top product PTRH2 Primary Antibody
  • Application Notes
    ELISA. Western Blot: Bit1 antibody can be used for the detection of Bit1 at 1 - 2 μg/mL. Immunohistochemistry.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Buffer
    PBS containing 0.02 % sodium azide.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C
    Storage Comment
    Store the antibody undiluted at 2-8 °C.
  • Target
    PTRH2 (Peptidyl-tRNA Hydrolase 2 (PTRH2))
    Alternative Name
    PTRH2 / BIT1 (PTRH2 Products)
    Synonyms
    BIT1 antibody, PTH2 antibody, A230072I16Rik antibody, Bit1 antibody, CGI-147 antibody, wu:fj09f02 antibody, zgc:109954 antibody, RGD1306819 antibody, peptidyl-tRNA hydrolase 2 antibody, ptrh2 antibody, CC1G_10259 antibody, ANI_1_1036184 antibody, AOR_1_2082154 antibody, PTRH2 antibody, Ptrh2 antibody
    Background
    Adhesion to extracellular matrix regulates cell survival through both integrin engagement and appropriate cell spreading. Anoikis is the molecular mechanism of apop-tosis induced by integrin detachment (1). Bit1 (Bcl-2 inhibitor of transcription 1) was recently identified as being involved in this process (2). Bit1 is a mitochondrial protein that is released into the cytoplasm upon onset of apoptosis where it forms a complex with AES, a small Groucho/transducin-like enhancer of split (TLE) protein and induces caspase-independent apoptosis. Both AES and TLE proteins are transcriptional co-repressors that play important roles in neurogenesis, segmentation, and sex determination (3). It has been suggested that Bit1-AES complexes turn off a survival-promoting gene transcription program controlled by TLE (2). Interestingly, apoptosis of cells transfected with Bit1 and AES could be inhibited if the cells were allowed to attach to fibronectin through the alpha5beta1 integrin suggesting that the Bit1-AES pathway contributing to anoikis is regulated by integrins, and in particular, the alpha5beta1 integrin (2).Synonyms: CGI-147, PTH2, Peptidyl-tRNA hydrolase 2 mitochondrial
    Gene ID
    51651
    NCBI Accession
    NP_057161
    UniProt
    Q9Y3E5
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