DACT2 antibody (N-Term)

Catalog No. ABIN499702

Product Details anti-DACT2 Antibody

Target: DACT2 (Dapper, Antagonist of beta-Catenin 2 (DACT2))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This DACT2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity: This antibody reacts to DACT2.

Purification: Affinity chromatography purified via peptide column

Immunogen: Dact2 antibody was raised against a 12 amino acid peptide from near the amino terminus of human DACT2.

Isotype: IgG

Application Details

Application Notes: ELISA. Western Blot: 1 - 2 μg/mL. Immunohistochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store the antibody undiluted at 2-8 °C.

References for anti-DACT2 antibody (ABIN499702)

Tan, Li, Huang, Cheng, Zhao, Chen, Chen, Tang, Zhang, Huang, Cheng: "Upregulation of DACT2 suppresses proliferation and enhances apoptosis of glioma cell via inactivation of YAP signaling pathway." in: Cell death & disease, Vol. 8, Issue 8, pp. e2981, (2018) (PubMed).

Zhao, Herman, Brock, Sheng, Zhang, Liu, Guo: "Methylation of DACT2 promotes papillary thyroid cancer metastasis by activating Wnt signaling." in: PLoS ONE, Vol. 9, Issue 11, pp. e112336, (2015) (PubMed).

Target Details for DACT2

Target: DACT2 (Dapper, Antagonist of beta-Catenin 2 (DACT2))

Alternative Name: DACT2 (DACT2 Products)

Synonyms: 2900084M21Rik antibody, A630024E20 antibody, Dpr2 antibody, Frd2 antibody, dapper2 antibody, C6orf116 antibody, DAPPER2 antibody, DPR2 antibody, RP11-503C24.7 antibody, bA503C24.7 antibody, dishevelled binding antagonist of beta catenin 2 antibody, dishevelled-binding antagonist of beta-catenin 2 antibody, DACT2 antibody, Dact2 antibody

Background: The Wnt signaling cascade is a conserved process in multicellular animals that plays important roles during development and can contribute to cancer and other diseases. Many members of this pathway are also expressed in the postnatal tissues such as brain. One such protein is Dact2, a member of the Dact protein family that was initially identified through binding to Disheveled (Dvl), a cytoplasmic protein essential to Wnt signaling. Dact2 is most prominent during the development of the thymus kidneys, and salivary gland. Dact2 is thought to play a role distinct from that of Dact1 with Dact2 having a greater impact on a b-catenin-independent process termed planar cell polarity/convergent-extension signaling. Furthermore, Dact2 but not Dact1 can inhibit Nodal signaling by promoting the endocytic degradation of TGF-b receptors. At least two isoforms of Dact2 are known to exist.Synonyms: C6orf116, DAPPER2, DPR2, PP13671, dapper homolog 2

Gene ID: 168002

NCBI Accession: NP_999627

UniProt: Q5SW24