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IRAK1 antibody (Interleukin-1 Receptor-Associated Kinase 1) (C-Term)

Details for Product anti-IRAK1 Antibody No. ABIN500036, Supplier: Log in to see
Antigen
  • IRAK1
  • IRAK
  • pelle
  • AA408924
  • IRAK-1
  • IRAK1-S
  • Il1rak
  • Plpk
  • mPLK
  • RGD1563841
Alternatives
anti-Human IRAK1 antibody for Immunofluorescence
Epitope
C-Term
37
33
22
17
15
14
13
13
13
13
13
9
8
7
6
5
5
5
5
4
4
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
221
111
78
Host
Rabbit
210
41
2
Clonality
Polyclonal
Conjugate
This IRAK1 antibody is un-conjugated
11
8
7
5
4
4
4
4
4
4
4
4
3
3
3
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Immunoprecipitation (IP), Western Blotting (WB)
174
107
52
45
44
39
28
19
16
8
8
3
2
Supplier
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Immunogen Human IRAK (C-Terminus) Peptide
Isotype IgG
Specificity IRAK antibody was raised against a peptide corresponding to amino acids near the carboxy terminus of human IRAK.
Purification DEAE
Alternative Name IRAK1 (IRAK1 Antibody Abstract)
Background Nuclear factor kappa B (NF-kappaB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-kappaB mediates the expression of a great variety of genes in response to extracellular stimuli including IL-1, TNFalpha and LPS. A serine/threonine protein kinase associated with IL-1 receptor (IRAK) and its homologue mouse pelle-like protein kinase (mPLK) were identified recently. IRAK is associated with the IL-1 receptor subunits IL-1RI and IL-1RAcP after IL-1 binding and serves as a signaling molecule to mediate IL-1 response. IRAK mediates a signaling cascade leading to NF-kappaB activation by members in IL-1 family including IL-1 and a novel cytokine IL-18 (also termed IGIF).Synonyms: IRAK, IRAK-1, Interleukin-1 receptor-associated kinase 1
Gene ID 3654
UniProt P51617
Research Area Cytokines, Receptors, Inflammation
Pathways NF-kappaB Signaling, TLR Signaling, Neurotrophin Signaling Pathway
Application Notes ELISA. Western Blot: 1/1000. Immunocytochemistry. Immunoprecipitation: 2 to 4 μg per sample. An 80 kDa band should be detected in the lysate from non-activated cells. It has no crossresponse to IRAK2.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Buffer PBS containing 0.02 % sodium azide.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C
Storage Comment Store the antibody undiluted at 2-8 °C.
Supplier Images
Immunofluorescence (IF) image for anti-IRAK1 antibody (Interleukin-1 Receptor-Associated Kinase 1) (C-Term) (ABIN500036) Immunocytochemistry of IRAK in HeLa cells with AP30438PU-N IRAK antibody at 10 μg/ml.
Western Blotting (WB) image for anti-IRAK1 antibody (Interleukin-1 Receptor-Associated Kinase 1) (C-Term) (ABIN500036) Western blot analysis of IRAK in THP-1 (THP) and HeLa (HL) whole cell lysates with AP...
Background publications Yao, Li, Shi: "Optically active supramolecular complexes of water-soluble achiral polythiophenes and folic acid: spectroscopic studies and sensing applications." in: Langmuir : the ACS journal of surfaces and colloids, Vol. 24, Issue 22, pp. 12829-35, 2008 (PubMed).

Huang, Gao, Li et al.: "Recruitment of IRAK to the interleukin 1 receptor complex requires interleukin 1 receptor accessory protein." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 94, Issue 24, pp. 12829-32, 1998 (PubMed).

Robinson, Shibuya, Mui et al.: "IGIF does not drive Th1 development but synergizes with IL-12 for interferon-gamma production and activates IRAK and NFkappaB." in: Immunity, Vol. 7, Issue 4, pp. 571-81, 1997 (PubMed).

Trofimova, Sprenkle, Green et al.: "Developmental and tissue-specific expression of mouse pelle-like protein kinase." in: The Journal of biological chemistry, Vol. 271, Issue 30, pp. 17609-12, 1996 (PubMed).

Cao, Henzel, Gao: "IRAK: a kinase associated with the interleukin-1 receptor." in: Science (New York, N.Y.), Vol. 271, Issue 5252, pp. 1128-31, 1996 (PubMed).