Insulin Receptor Substrate 1 (IRS1) (Center) antibody

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Antigen
Synonyms irs1, irsu, IRS1, HIRS-1, G972R, IRS-1, IRS1IRM, irs-1, irs1-a
Alternatives
Epitope
Center
(52), (51), (49), (48), (32), (26), (24), (21), (16), (15), (15), (14), (13), (13), (13), (13), (13), (13), (11), (8), (7), (7), (6), (6), (6), (5), (4), (4), (3), (3), (3), (3), (3), (3), (3), (3), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine)
(589), (392), (367), (6), (5), (4), (1), (1), (1)
Host
Rabbit
(588), (35)
Clonality
Polyclonal
Conjugate
Un-conjugated
(13), (12), (11), (11), (11), (10), (10), (10), (10), (10), (10), (10), (2), (1), (1), (1)
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
(376), (255), (182), (112), (112), (95), (34), (30), (10), (3), (3), (2)
Pubmed 4 references available
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Quantity 0.1 mg
Shipping to United States ( )
Availability Will be delivered in 6 to 8 Business Days
Immunogen IRS-1 antibody was raised against a 16 amino acid peptide from near the center of human IRS-1.
Isotype IgG
Specificity This antibody detects IRS-1.
Cross-Reactivity (Details) Species reactivity (tested):Human, mouse
Purification Peptide affinity chromatography
Alternative Name IRS1 (IRS1 Antibody Abstract)
Background Following tyrosine phosphorylation, the insulin receptor substrate 1 and 2 (IRS-1 and IRS-2) can form a protein scaffolding for the assembly of a host of Src homology 2 (SH2) domain-containing proteins (1). IRS-1 tyrosine phosphorylation can occur through the activity of several cytokine and growth factor receptors such as interleukin (IL)-4, IL-9, interferon-?, in addition to the insulin and insulin-like growth factor 1 receptors (2). The scaffolding provided by IRS-1 and IRS-2 is necessary for insulin signal transduction across the cell membrane. IRS-1 tyrosine phosphorylation, and thus formation of the IRS scaffolding is inhibited by tumor necrosis factor (TNF), and this inhibition can itself be blocked by rapamycin, an inhibitor of the mammalian target of rapamycin (TOR) (3,4). TNF activity could also be blocked by inhibition of the Akt kinase and the PTEN tumor suppressor, suggesting that TNF impairs insulin signaling through IRS-1 by activation of the TOR signaling pathway (3).Synonyms: IRS-1, Insulin receptor substrate 1
Gene ID 3667
UniProt P35568
Research Area Cardiovascular, Atherosclerosis, Signaling, Growth Factors, Cancer
Pathways Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway
Application Notes ELISA. Western blot: 1 - 2 μg/mL. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Buffer PBS containing 0.02 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.
Supplier Images
Immunofluorescence (IF) image for anti-Insulin Receptor Substrate 1 (IRS1) (Center) antibody (ABIN500072) Immunocytochemistry of IRS-1 in P815 cells with this product at 2 μg/ml.
Western Blotting (WB) image for anti-Insulin Receptor Substrate 1 (IRS1) (Center) antibody (ABIN500072) Western blot analysis of IRS-1 in PC-3 cell lysate with this product at (A) 1, (B) 2 ...
Background publications Waters, Pessin: "Insulin receptor substrate 1 and 2 (IRS1 and IRS2): what a tangled web we weave." in: Trends in cell biology, Vol. 6, Issue 1, pp. 1-4, 2004 (PubMed).

Shamji, Nghiem, Schreiber: "Integration of growth factor and nutrient signaling: implications for cancer biology." in: Molecular cell, Vol. 12, Issue 2, pp. 271-80, 2003 (PubMed).

Ozes, Akca, Mayo et al.: "A phosphatidylinositol 3-kinase/Akt/mTOR pathway mediates and PTEN antagonizes tumor necrosis factor inhibition of insulin signaling through insulin receptor substrate-1." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 8, pp. 4640-5, 2001 (PubMed).

Giovannone, Scaldaferri, Federici et al.: "Insulin receptor substrate (IRS) transduction system: distinct and overlapping signaling potential." in: Diabetes/metabolism research and reviews, Vol. 16, Issue 6, pp. 434-41, 2001 (PubMed).

Catalog No. ABIN500072
$401.50
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