PAFAH1B1 antibody (C-Term)

Catalog No. ABIN500180

Product Details anti-PAFAH1B1 Antibody

Target: PAFAH1B1 (Platelet-Activating Factor Acetylhydrolase 1b, Regulatory Subunit 1 (45kDa) (PAFAH1B1))

Binding Specificity: C-Term

Reactivity: Human, Rat, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PAFAH1B1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Enzyme Immunoassay (EIA)

Specificity: This antibody detects PAFAH1B1 / LIS1.

Purification: Peptide affinity chromatography

Immunogen: LIS1 antibody was raised against a 14 amino acid peptide from near the carboxy terminus of human LIS1.

Isotype: IgG

Application Details

Application Notes: ELISA. Western blot: 0.5 - 1 μg/mL. Immunoflorescence.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Buffer: PBS containing 0.02 % sodium azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C

Storage Comment: Store at 2 - 8 °C.

Target Details for PAFAH1B1

Target: PAFAH1B1 (Platelet-Activating Factor Acetylhydrolase 1b, Regulatory Subunit 1 (45kDa) (PAFAH1B1))

Alternative Name: PAFAH1B1 / LIS1 (PAFAH1B1 Products)

Background: Lissencephaly is a severe brain developmental disease characterized by the mislocalization of cortical neurons, a smooth cerebral surface, mental retardation, and seizures. Classical lissencephaly is caused by sporadic mutations in the LIS1 gene. While LIS1 is known to act in a pathway deactivating the lipid messenger platelet-activating factor, LIS1 forms a complex with Nudel and 14-3-3e which is then transported from neuronal cell bodies through the actions of DISC1 and KIF5A, a microtubule-dependent directed motor protein kinesin. Decreased expression of LIS1 blocked neural stem cell division, morphogenesis, and motility, suggesting that LIS1 plays an important role in neuronal cell proliferation and localization in the developing brain. At least two isoforms of LIS1 are known to exist.Synonyms: LIS-1, Lissencephaly-1 protein, MDCR, MDS, PAF acetylhydrolase 45 kDa subunit, PAF-AH alpha, PAFAH alpha, PAFAHA, Platelet-activating factor acetylhydrolase IB subunit alpha

Gene ID: 5048

UniProt: P43034

Pathways: M Phase, Regulation of Cell Size