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TANK-Binding Kinase 1 (TBK1) (C-Term) antibody

Details for Product No. ABIN500308, Supplier: Log in to see
Antigen
  • 1200008B05Rik
  • AI462036
  • AW048562
  • NAK
  • nak
  • t2k
  • T2K
  • TBK1
  • wu:fk70c05
  • zgc:136548
Alternatives
anti-Human TANK-Binding Kinase 1 antibody for Western Blotting
Epitope
C-Term
28
22
22
15
12
7
3
3
2
2
2
2
2
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
134
75
44
23
23
4
4
2
2
2
2
2
1
1
1
1
1
Host
Rabbit
105
39
10
Clonality
Polyclonal
Conjugate
Un-conjugated
10
9
9
9
6
6
3
3
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
128
66
50
35
12
7
6
4
3
3
2
2
1
1
Supplier
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Immunogen Peptide from C-term of human NAK / TBK1 (Cat. No. 2351P)
Isotype IgG
Specificity This antibody detects TBK1 at C-term. It has no cross response to IKKa, IKKb, IKKy, or IKKe.
Cross-Reactivity (Details) Species reactivity (tested):Human, mouse
Purification Peptide affinity chromatography
Alternative Name TBK1 (TBK1 Antibody Abstract)
Background Nuclear factor kappa B (NF-kappaB) is a ubiquitous transcription factor and an essential mediator of gene expression during activation of immune and inflammatory responses. NF-kappaB mediates the expression of a great variety of genes in response to extracellular stimuli. NF-kappaB is associated with IkappaB proteins in the cell cytoplasm, which inhibit NF-kappaB activity. Phosphorylation of I-kappaB by IkappaB kinase (IKK) complex leads to degradation of I-kappaB and activation of NF-kappaB. The IKK complex contains IKKalpha, IKKbeta, and IKKgamma. A novel IKK related kinase was recently identified and designated TBK1 (TANK-binding kinase 1), NAK (NF-kappaB-activating kinase), and T2K (1-3). NAK/TBK1 activates IKKbeta through direct phosphorylation. NAK/TBK1 is activated by growth factors and PMA and mediates IKK and NF-kappaB activation in response to growth factors (2). NAK/TBK1 functions upstream of NIK and the IKK complex (1,2). NAK/TBK1 is also critical in protecting embryonic liver from apoptosis (3).Synonyms: NAK, NF-kappa-B-activating kinase, T2K, TANK-binding kinase 1
Gene ID 29110
NCBI Accession NP_037386
UniProt Q9UHD2
Pathways TLR Signaling
Application Notes ELISA. Western blot: 0.5 - 1 μg/mL, an 84 kDa band should be detected. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Buffer PBS containing 0.02 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.
Supplier Images
Immunofluorescence (IF) image for anti-TANK-Binding Kinase 1 (TBK1) (C-Term) antibody (ABIN500308) Immunocytochemistry of NAK in MOLT4 cells with this product at 10 μg/ml.
Background publications Angelone, Quintieri, Amodio, Cerra: "Endocrine orchestration of cardiovascular, gastrointestinal and hypothalamic control." in: Current medicinal chemistry, Vol. 18, Issue 32, pp. 4976-86, 2011 (PubMed).

Rao, Kadesch: "The intracellular form of notch blocks transforming growth factor beta-mediated growth arrest in Mv1Lu epithelial cells." in: Molecular and cellular biology, Vol. 23, Issue 18, pp. 6694-701, 2003 (PubMed).

Pomerantz, Baltimore: "NF-kappaB activation by a signaling complex containing TRAF2, TANK and TBK1, a novel IKK-related kinase." in: The EMBO journal, Vol. 18, Issue 23, pp. 6694-704, 2000 (PubMed).

Bonnard, Mirtsos, Suzuki, Graham, Huang, Ng, Itié, Wakeham, Shahinian, Henzel, Elia, Shillinglaw, Mak, Cao, Yeh: "Deficiency of T2K leads to apoptotic liver degeneration and impaired NF-kappaB-dependent gene transcription." in: The EMBO journal, Vol. 19, Issue 18, pp. 4976-85, 2000 (PubMed).

Tojima, Fujimoto, Delhase, Chen, Hatakeyama, Nakayama, Kaneko, Nimura, Motoyama, Ikeda, Karin, Nakanishi: "NAK is an IkappaB kinase-activating kinase." in: Nature, Vol. 404, Issue 6779, pp. 778-82, 2000 (PubMed).