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NLRP3 antibody (NLR Family, Pyrin Domain Containing 3) (N-Term)

Details for Product anti-NLRP3 Antibody No. ABIN500314, Supplier: Log in to see
Antigen
  • CIAS1
  • Nalp3
  • avp
  • cias1
  • nalp3
  • pypaf1
  • agtavprl
  • AGTAVPRL
  • AII
  • AVP
  • C1orf7
  • CLR1.1
  • FCAS
  • FCU
  • MWS
  • NALP3
  • PYPAF1
  • AII/AVP
  • Cias1
  • Mmig1
  • Pypaf1
Epitope
N-Term
37
28
16
14
6
5
4
3
3
3
2
2
2
2
2
1
1
Reactivity
Human, Mouse (Murine)
159
34
29
6
5
4
2
1
1
Host
Rabbit
84
50
29
3
Clonality
Polyclonal
Conjugate
This NLRP3 antibody is un-conjugated
6
6
6
5
5
5
2
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
103
84
30
26
17
13
9
8
6
4
3
2
1
Supplier
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Immunogen A 16 amino acid peptide from near the amino terminus of Human NALP3.
Isotype IgG
Cross-Reactivity (Details) Species reactivity (tested):Human, Mouse
Purification Paptide Affinity Chromatography
Alternative Name NALP3 (NLRP3 Antibody Abstract)
Background NALP3, a member of Nod-like receptors, has a crucial role in inflammation and immunity and may be a proximal sensor of cellular stress and danger signals. NALP3 forms a caspase-1 activating molecular complex termed the inflammasome. The inflammasome allows activation of IL-1b, the key player of the inflammation and fever. NALP3 gene encodes a pyrin like protein which contains a pyrin domain, a nucleotide binding site (NBS) domain, and a leucine rich repeat (LRR) motif. NALP3 protein interacts with apoptosis associated speck like protein containing a CARD and may function as an inducer of apoptosis and an activator of NF-kB signaling. Defects in NALP3 have been associated with familial cold autoinflammatory syndrome (FCAS), Muckle-Wells syndrome (MWS), chronic infantile neurological cutaneous and articular (CINCA) syndrome, and neonatal-onset multisystem inflammatory disease (NOMID).Synonyms: C1orf7, CIAS1, LRR and PYD domains-containing protein 3, NACHT, NLRP3, PYPAF1
Gene ID 114548
NCBI Accession NP_899632
UniProt Q96P20
Pathways
Application Notes ELISA. Western blot: 1 - 2 μg/mL. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Concentration 1.0 mg/mL
Buffer PBS, 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage -20 °C
Storage Comment Store the antibody (in aliquots) at -20 °C.
Supplier Images
Immunofluorescence (IF) image for anti-NLRP3 antibody (NLR Family, Pyrin Domain Containing 3) (N-Term) (ABIN500314) Immunofluorescence of NALP3 in K562 cells with NALP3 antibody at 20 μg/ml.
Western Blotting (WB) image for anti-NLRP3 antibody (NLR Family, Pyrin Domain Containing 3) (N-Term) (ABIN500314) Western blot analysis of NALP3 in K562 cell lysate with NALP3 antibody at 1 μg/ml (A)...
Background publications Mohamad, Surikumaran, Raoov et al.: "Conventional Study on Novel Dicationic Ionic Liquid Inclusion with β-Cyclodextrin." in: International journal of molecular sciences, Vol. 12, Issue 9, pp. 6329-45, 2011 (PubMed).

Kanneganti, Ozören, Body-Malapel et al.: "Bacterial RNA and small antiviral compounds activate caspase-1 through cryopyrin/Nalp3." in: Nature, Vol. 440, Issue 7081, pp. 233-6, 2006 (PubMed).

Agostini, Martinon, Burns et al.: "NALP3 forms an IL-1beta-processing inflammasome with increased activity in Muckle-Wells autoinflammatory disorder." in: Immunity, Vol. 20, Issue 3, pp. 319-25, 2004 (PubMed).

OConnor, Harton, Zhu et al.: "Cutting edge: CIAS1/cryopyrin/PYPAF1/NALP3/CATERPILLER 1.1 is an inducible inflammatory mediator with NF-kappa B suppressive properties." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 171, Issue 12, pp. 6329-33, 2003 (PubMed).

Chamaillard, Girardin, Viala et al.: "Nods, Nalps and Naip: intracellular regulators of bacterial-induced inflammation." in: Cellular microbiology, Vol. 5, Issue 9, pp. 581-92, 2003 (PubMed).