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Nicastrin antibody (Center)

NCSTN Reactivity: Human, Mouse, Rat WB, IHC (p), EIA Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN500345
  • Target See all Nicastrin (NCSTN) Antibodies
    Nicastrin (NCSTN)
    Binding Specificity
    • 15
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    Center
    Reactivity
    • 37
    • 32
    • 21
    • 6
    • 6
    • 5
    • 1
    • 1
    Human, Mouse, Rat
    Host
    • 51
    • 2
    Rabbit
    Clonality
    • 43
    • 10
    Polyclonal
    Conjugate
    • 20
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This Nicastrin antibody is un-conjugated
    Application
    • 39
    • 13
    • 13
    • 9
    • 6
    • 4
    • 4
    • 3
    • 3
    • 2
    • 1
    Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
    Specificity
    This antibody detects Nicastrin at center.
    Cross-Reactivity (Details)
    Species reactivity (tested):Human, mouse, rat
    Purification
    Peptide affinity chromatography
    Immunogen
    Nicastrin antibody was raised against a 18 amino acid peptide from near the center of human Nicastrin.
    Isotype
    IgG
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  • Application Notes
    ELISA. Western blot: 0.5 - 1 μg/mL. Immunohistochemistry on paraffin sections.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Buffer
    PBS containing 0.02 % sodium azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.
  • Target
    Nicastrin (NCSTN)
    Alternative Name
    Nicastrin (NCSTN Products)
    Synonyms
    CG7012 antibody, Dmel\\CG7012 antibody, Nct antibody, Nic antibody, S5A antibody, ST96B antibody, agro antibody, dNct antibody, nic antibody, NCSTN antibody, DKFZp469O2418 antibody, APH2 antibody, 9430068N19Rik antibody, AA727311 antibody, Aph2 antibody, D1Dau13e antibody, Kiaa0253 antibody, mKIAA0253 antibody, nicastrin antibody, Nicastrin antibody, NCSTN antibody, nct antibody, ncstn antibody, CpipJ_CPIJ001617 antibody, Ncstn antibody, aph-2 antibody
    Background
    Nicastrin, in addition to presenilin, PEN2, and APH-1 forms the γ-secretase protein complex, a membrane-bound aspartyl protease that can cleave certain proteins at peptide bonds buried within the hydrophobic environment of the lipid bilayer. This cleavage is responsible for a key step in signaling from several cell-surface receptors and is thought to be required for the generation of the neurotoxic amyloid peptides that are central to the pathogenesis of Alzheimer's disease. Like the tumor necrosis factor-a-converting enzyme (TACE) and the b-site cleavage enzyme (BACE) protease families, γ-secretase will cleave the amyloid precursor protein (APP), but within the intramembrane region of APP, resulting in either the non-toxic p3 (from the α and γ cleavage site) or the toxic Aβ amyloid peptide (from the β and γ cleavage site). It is thought that accumulation of the Aβ peptide is the precursor to Alzheimer's disease. Nicastrin is also thought to be involved in cell proliferation and signaling, especially in regards to activation of Notch receptors as loss of Nicastrin expression results in mouse embryonic lethality.Synonyms: KIAA0253, NCSTN, UNQ1874/PRO4317
    Gene ID
    23385
    NCBI Accession
    NP_056146
    UniProt
    Q92542
    Pathways
    Notch Signaling, Neurotrophin Signaling Pathway
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