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NOD2 antibody (Nucleotide-Binding Oligomerization Domain Containing 2) (N-Term)

Details for Product anti-NOD2 Antibody No. ABIN500363, Supplier: Log in to see
Antigen
  • ACUG
  • BLAU
  • CARD15
  • CD
  • CLR16.3
  • IBD1
  • NLRC2
  • NOD2B
  • PSORAS1
  • Card15
  • F830032C23Rik
  • Nlrc2
  • caspase recruitment domain 15 protein
  • nucleotide-binding oligomerization domain containing 2
  • TTHERM_01951320
  • NOD2
  • Nod2
Alternatives
anti-Human NOD2 antibody for Flow Cytometry
Epitope
N-Term
36
25
12
12
11
5
4
3
1
1
1
Reactivity
Human
145
52
20
1
1
Host
Rabbit
95
33
17
6
Clonality
Polyclonal
Conjugate
This NOD2 antibody is un-conjugated
6
6
4
4
4
4
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
126
47
46
43
37
36
30
23
13
5
3
2
2
Options
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Immunogen Rabbit NOD2 polyclonal antibody was raised against a synthetic peptide corresponding to 16 amino acids at the amino terminus of human NOD2.
Isotype IgG
Specificity This antibody detects CARD15 / NOD2 at N-term.
Cross-Reactivity (Details) Species reactivity (tested):ELISA.Western blot: 2 to 4 μg/mL.Immunohistochemistry on paraffin sections.
Purification Peptide affinity chromatography
Alternative Name CARD15 / NOD2 (NOD2 Antibody Abstract)
Background Apaf-1 and NOD1 are members of a new family (1), which are involved in the regulation of apoptosis and immune response. Each of them contains a caspase recruitment domain (CARD) and a nucleotide-binding oligomerization domain (NOD). A third member in this family was recently identified and designated NOD2 (2). NOD2 interacts with RICK via a homophilic CARD-CARD interaction. NOD2 activates NF-κB, which is regulated by its carboxy-terminal leucine-rich repeat domain that acts as an intracellular receptor for components of bacteria. The variants of NOD2, either a frameshift or a missense, were associated with Crohn's disease (3,4) that is a main type of chronic inflammatory bowel disease.Synonyms: Caspase recruitment domain-containing protein 15, IBD1, Inflammatory bowel disease protein 1, Nucleotide-binding oligomerization domain-containing protein 2
Gene ID 64127
UniProt Q9HC29
Research Area Signaling, Transcription Factors
Pathways Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity
Application Notes ELISA. Western blot: 2 to 4 μg/mL. Immunohistochemistry on paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Buffer PBS containing 0.02 % sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.
Supplier Images
Immunofluorescence (IF) image for anti-NOD2 antibody (Nucleotide-Binding Oligomerization Domain Containing 2) (N-Term) (ABIN500363) Immunocytochemistry of NOD2 in Jurkat cells with this product at 5 μg/ml.
Western Blotting (WB) image for anti-NOD2 antibody (Nucleotide-Binding Oligomerization Domain Containing 2) (N-Term) (ABIN500363) Western blot analysis of NOD2 in Jurkat cell lysate with this product at (A) 1, (B) 2...
Background publications Ogura, Inohara, Benito, Chen, Yamaoka, Nunez: "Nod2, a Nod1/Apaf-1 family member that is restricted to monocytes and activates NF-kappaB." in: The Journal of biological chemistry, Vol. 276, Issue 7, pp. 4812-8, 2001 (PubMed).

Hugot, Chamaillard, Zouali, Lesage, Cézard, Belaiche, Almer, Tysk, OMorain, Gassull, Binder, Finkel, Cortot, Modigliani, Laurent-Puig, Gower-Rousseau, Macry, Colombel, Sahbatou, Thomas: "Association of NOD2 leucine-rich repeat variants with susceptibility to Crohn's disease." in: Nature, Vol. 411, Issue 6837, pp. 599-603, 2001 (PubMed).

Ogura, Bonen, Inohara, Nicolae, Chen, Ramos, Britton, Moran, Karaliuskas, Duerr, Achkar, Brant, Bayless, Kirschner, Hanauer, Nuñez, Cho: "A frameshift mutation in NOD2 associated with susceptibility to Crohn's disease." in: Nature, Vol. 411, Issue 6837, pp. 603-6, 2001 (PubMed).

Inohara, Koseki, del Peso, Hu, Yee, Chen, Carrio, Merino, Liu, Ni, Núñez: "Nod1, an Apaf-1-like activator of caspase-9 and nuclear factor-kappaB." in: The Journal of biological chemistry, Vol. 274, Issue 21, pp. 14560-7, 1999 (PubMed).