NOXA antibody (N-Term)

Catalog No. ABIN500377

Product Details anti-NOXA Antibody

Target: NOXA (PMAIP1) (Phorbol-12-Myristate-13-Acetate-Induced Protein 1 (PMAIP1))

Binding Specificity: N-Term

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NOXA antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Specificity: This antibody detects NOXA.

Purification: Peptide affinity chromatography

Immunogen: Noxa antibody was raised against a synthetic peptide corresponding to 17 amino acids at the amino terminus of mouse Noxa.

Isotype: IgG

Application Details

Application Notes: ELISA. Western blot. Immunohistochemistry on paraffin sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 0,5 mg/mL

Buffer: PBS containing 0.02 % sodium azide as preservative

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for NOXA

Target: NOXA (PMAIP1) (Phorbol-12-Myristate-13-Acetate-Induced Protein 1 (PMAIP1))

Alternative Name: NOXA (PMAIP1 Products)

Background: Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes including p53R2, p53AIP1, and PUMA. A new p53 target gene, Noxa, was recently identified , which encodes a protein belonging to the subfamily of BH3-only proapoptic proteins. Noxa and PUMA are both transcriptional targets of p53 and BH3-only proteins. X-ray irradiation increased p53-dependent Noxa mRNA and protein levels. Noxa, when ectopically expressed, interacted with anti-apoptotic Bcl-2 family members, resulting in the activation of caspase-9 . Noxa, like PUMA, localized to mitochondria and induces apoptosis in response to p53 (1-3). Noxa and PUMA may represent direct mediators of p53-induced apoptosis. Increased levels of p53 and its target gene Noxa was found in the impaired tumor development (4).Synonyms: PMA-induced protein 1, PMAIP1, Phorbol-12-myristate-13-acetate-induced protein 1

Gene ID: 58801

NCBI Accession: NP_067426

UniProt: Q9JM54

Pathways: p53 Signaling, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus