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RRM2B antibody (Ribonucleotide Reductase M2 B (TP53 Inducible)) (AA 2-17)

Details for Product anti-RRM2B Antibody No. ABIN500437, Supplier: Log in to see
Antigen
  • MTDPS8A
  • MTDPS8B
  • P53R2
  • p53r2
  • p53R2
  • RRM2B
Alternatives
anti-Human RRM2B antibody for Western Blotting
Epitope
AA 2-17, N-Term
17
16
15
12
10
3
3
2
2
2
2
1
1
1
Reactivity
Human, Mouse (Murine), Rat (Rattus)
87
38
35
2
1
Host
Rabbit
82
5
Clonality
Polyclonal
Conjugate
This RRM2B antibody is un-conjugated
3
3
3
2
2
2
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
67
41
19
13
11
9
4
3
1
Supplier
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Immunogen p53R2 antibody was raised against a synthetic peptide corresponding to amino acids 2 to 17 of human p53R2.
Isotype IgG
Specificity This antibody detects p53R2 at N-term.
Purification Peptide Affinity Chromatography
Alternative Name RRM2B / P53R2 (RRM2B Antibody Abstract)
Background The p53 tumor-suppresspr gene integrates numerous signals that control cell life and death. Several novel molecules involved in p53 signaling, including p53R2 , Chk2 (2), p53AIP1 (3), Noxa (4), PIDD (5), and PID/MTA2 (6), were recently discovered. p53R2 is a p53 inducible gene that contains a p53 binding sequence and encodes a subunit of the enzyme ribonucleotide reductase (1,7). p53R2 is induced by the reagents, ultraviolet and g-irradiation that cause DNA damages. The product of p53R2 gene is directly involved in the p53 checkpoint for repair of damaged DNA. The isoform of the p53 family member p73 also induces p53R2 expression (8). p53R2 is an important target of p53 for tumour suppression.Synonyms: Ribonucleoside-diphosphate reductase subunit M2 B, TP53-inducible ribonucleotide reductase M2 B, p53-inducible ribonucleotide reductase small subunit 2-like protein
Gene ID 50484
UniProt Q7LG56
Pathways p53 Signaling
Application Notes ELISA. Western blot: 0.5-1 μg/mL (A 39 kDa band can be detected). Immunohistochemistry on Paraffin Sections. Positive Controls: A431 Cell Lysate, 3T3 Whole Cell Lysate, Human Lung Tissue Lysate.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Buffer PBS containing 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) image for anti-RRM2B antibody (Ribonucleotide Reductase M2 B (TP53 Inducible)) (AA 2-17) (ABIN500437) AP30640PU-N p53R2 antibody staining of Human lung tissue by Immunohistochemistry at 1...
Western Blotting (WB) image for anti-RRM2B antibody (Ribonucleotide Reductase M2 B (TP53 Inducible)) (AA 2-17) (ABIN500437) Western blot analysis of p53R2 expression in A431 cell lysate with this product at (A...
Background publications Tanaka, Arakawa, Yamaguchi, Shiraishi, Fukuda, Matsui, Takei, Nakamura: "A ribonucleotide reductase gene involved in a p53-dependent cell-cycle checkpoint for DNA damage." in: Nature, Vol. 404, Issue 6773, pp. 42-9, 2000 (PubMed).

Oda, Ohki, Murasawa, Nemoto, Shibue, Yamashita, Tokino, Taniguchi, Tanaka: "Noxa, a BH3-only member of the Bcl-2 family and candidate mediator of p53-induced apoptosis." in: Science (New York, N.Y.), Vol. 288, Issue 5468, pp. 1053-8, 2000 (PubMed).

Oda, Arakawa, Tanaka, Matsuda, Tanikawa, Mori, Nishimori, Tamai, Tokino, Nakamura, Taya: "p53AIP1, a potential mediator of p53-dependent apoptosis, and its regulation by Ser-46-phosphorylated p53." in: Cell, Vol. 102, Issue 6, pp. 849-62, 2000 (PubMed).

Matsuoka, Huang, Elledge: "Linkage of ATM to cell cycle regulation by the Chk2 protein kinase." in: Science (New York, N.Y.), Vol. 282, Issue 5395, pp. 1893-7, 1998 (PubMed).