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BBC3 antibody (BCL2 Binding Component 3) (C-Term)

Details for Product anti-BBC3 Antibody No. ABIN500557, Supplier: Login to see
Antigen
  • BBC3
  • Puma
  • JFY-1
  • JFY1
  • PUMA
  • PUMA/JFY1
Alternatives
anti-Human BBC3 antibody for Immunohistochemistry (Paraffin-embedded Sections)
Epitope
C-Term
89
43
21
13
13
11
8
7
6
3
3
2
2
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
253
73
55
2
1
1
Host
Rabbit
225
25
13
Clonality
Polyclonal
Conjugate
This BBC3 antibody is un-conjugated
10
9
7
7
7
5
3
3
3
3
3
3
3
3
3
3
3
1
1
1
1
1
1
1
1
1
1
1
1
1
1
Application
Enzyme Immunoassay (EIA), Immunofluorescence (IF), Western Blotting (WB)
240
71
55
46
36
33
11
8
7
6
3
2
2
Supplier
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Immunogen Synthetic peptide corresponding to 14 amino acids near the carboxy terminus of Human PUMA alpha. .Remarks: The sequence is identical between alpha and beta forms of the PUMA proteins.
Isotype IgG
Specificity This antibody detects PUMA at C-term.
Cross-Reactivity (Details) Species reactivity (tested):Human, Mouse.
Purification Peptide Affinity Chromatography
Alternative Name PUMA (BBC3 Antibody Abstract)
Background Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse (1-3). PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-alpha and PUMA-beta (1). PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.Synonyms: BBC3, Bcl-2-binding component 3, JFY-1, p53 up-regulated modulator of apoptosis
Molecular Weight 23 kDa
Gene ID 27113
NCBI Accession NP_055232
UniProt Q9BXH1
Research Area Cancer, Apoptosis/Necrosis, Metabolism
Pathways p53 Signaling
Application Notes ELISA. Western blot: 1 - 2 μg/mL. K562 or 3T3 cell lysate can be used as a positive control and aband at approximately 23 kDa can be detected. A lower band at approximately 16 kDa wasdetected in MOLT4 and U937 cells, which may represent the PUMA-b form. Immunocytochemistry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Buffer PBS, 0.02 % Sodium Azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
Supplier Images
Immunofluorescence (IF) image for anti-BBC3 antibody (BCL2 Binding Component 3) (C-Term) (ABIN500557) Immunocytochemistry of PUMA in K562 cells with this product at 1 µg/ml.
Immunofluorescence (IF) image for anti-BBC3 antibody (BCL2 Binding Component 3) (C-Term) (ABIN500557) Immunofluorescence of PUMA in K562 cells with this product at 2 µg/ml.
Western Blotting (WB) image for anti-BBC3 antibody (BCL2 Binding Component 3) (C-Term) (ABIN500557) Western blot analysis of PUMA expression in human (H) K562 and mouse (M) 3T3 cell lys...
Background publications Han, Flemington, Houghton et al.: "Expression of bbc3, a pro-apoptotic BH3-only gene, is regulated by diverse cell death and survival signals." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 20, pp. 11318-23, 2001 (PubMed).

Nakano, Vousden: "PUMA, a novel proapoptotic gene, is induced by p53." in: Molecular cell, Vol. 7, Issue 3, pp. 683-94, 2001 (PubMed).

Yu, Zhang, Hwang et al.: "PUMA induces the rapid apoptosis of colorectal cancer cells." in: Molecular cell, Vol. 7, Issue 3, pp. 673-82, 2001 (PubMed).