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BCL2 Binding Component 3 (BBC3) (C-Term) antibody

Details for Product No. ABIN500557
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Antigen
Synonyms BBC3, Puma, JFY-1, JFY1, PUMA, PUMA/JFY1
Epitope
C-Term
(62), (37), (12), (6), (5), (4), (2), (2), (2), (1), (1), (1), (1), (1), (1)
Reactivity
Human, Mouse (Murine)
(200), (54), (53), (12), (12), (3)
Host
Rabbit
(165), (41), (3)
Clonality
Polyclonal
Conjugate
Un-conjugated
(5), (4), (4), (4), (4), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1), (1)
Application
ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
(194), (69), (45), (37), (26), (19), (10), (5), (5), (2)
Pubmed 3 references available
Quantity 0.1 mg
Shipping to United States (Change)
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Catalog No. ABIN500557
401.50 $
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Immunogen Synthetic peptide corresponding to 14 amino acids near the carboxy terminus of Human PUMA alpha. .Remarks: The sequence is identical between alpha and beta forms of the PUMA proteins.
Isotype IgG
Specificity This antibody detects PUMA at C-term. Species Reactivity: Tested: Human, Mouse.
Purification Peptide Affinity Chromatography
Alternative Name PUMA
Background Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse (1-3). PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-alpha and PUMA-beta (1). PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.
Alternate names: BBC3, Bcl-2-binding component 3, JFY-1, p53 up-regulated modulator of apoptosis
Gene ID 27113
NCBI Accession NP_055232
UniProt Q9BXH1
Research Area Cancer, Apoptosis/Necrosis, Metabolism
Application Notes ELISA. Western blot: 1 - 2 μg/ml. K562 or 3T3 cell lysate can be used as a positive control and aband at approximately 23 kDa can be detected. A lower band at approximately 16 kDa wasdetected in MOLT4 and U937 cells, which may represent the PUMA-b form. Immunocytochemistry. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Buffer PBS, 0.02% Sodium azide
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Expiry Date 12 months
Background publications Yu, Zhang, Hwang et al.: "PUMA induces the rapid apoptosis of colorectal cancer cells." in: Molecular cell, Vol. 7, Issue 3, pp. 673-82, 2001 (PubMed).

Nakano, Vousden: "PUMA, a novel proapoptotic gene, is induced by p53." in: Molecular cell, Vol. 7, Issue 3, pp. 683-94, 2001 (PubMed).

Han, Flemington, Houghton et al.: "Expression of bbc3, a pro-apoptotic BH3-only gene, is regulated by diverse cell death and survival signals." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 20, pp. 11318-23, 2001 (PubMed).

Validation Images
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