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PUMA antibody (N-Term)

BBC3 Reactivity: Human WB, IF, IHC (p), EIA Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN500559
  • Target See all PUMA (BBC3) Antibodies
    PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))
    Binding Specificity
    • 21
    • 17
    • 16
    • 10
    • 8
    • 7
    • 6
    • 6
    • 6
    • 6
    • 4
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    N-Term
    Reactivity
    • 113
    • 40
    • 39
    • 16
    Human
    Host
    • 109
    • 7
    • 1
    Rabbit
    Clonality
    • 98
    • 19
    Polyclonal
    Conjugate
    • 52
    • 8
    • 8
    • 6
    • 5
    • 5
    • 3
    • 3
    • 3
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    This PUMA antibody is un-conjugated
    Application
    • 98
    • 40
    • 39
    • 22
    • 22
    • 16
    • 16
    • 14
    • 13
    • 9
    • 4
    • 3
    • 2
    • 2
    Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
    Specificity
    This antibody detects PUMA at N-term.
    Cross-Reactivity (Details)
    Species reactivity (tested):Human.
    Purification
    Peptide Affinity Chromatography
    Immunogen
    Synthetic peptide corresponding to 14 amino acids near the amino terminus of Human PUMA-alpha. .Remarks: This sequence is identical between alpha and beta forms of the PUMA proteins.
    Isotype
    IgG
    Top Product
    Discover our top product BBC3 Primary Antibody
  • Application Notes
    ELISA. Western blot: 2 to 4 μg/mL, K562 cell lysate can be used as a positive control and a band atapproximately 23 kDa can be detected. Immunohistochemistry on paraffin sections.
    Other applications not tested.
    Optimal dilutions are dependent on conditions and should be determined by the user.
    Restrictions
    For Research Use only
  • Buffer
    PBS, 0.02 % Sodium Azide
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Handling Advice
    Avoid repeated freezing and thawing.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.
  • Target
    PUMA (BBC3) (BCL2 Binding Component 3 (BBC3))
    Alternative Name
    PUMA (BBC3 Products)
    Synonyms
    BBC3 antibody, Puma antibody, JFY-1 antibody, JFY1 antibody, PUMA antibody, PUMA/JFY1 antibody, BCL2 binding component 3 antibody, Bcl-2 binding component 3 antibody, BBC3 antibody, Bbc3 antibody, bbc3 antibody
    Background
    Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse (1-3). PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-alpha and PUMA-beta (1). PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.Synonyms: BBC3, Bcl-2-binding component 3, JFY-1, p53 up-regulated modulator of apoptosis
    Gene ID
    27113
    NCBI Accession
    NP_055232
    UniProt
    Q9BXH1
    Pathways
    p53 Signaling, Positive Regulation of Endopeptidase Activity
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