Search, Find and Buy Antibodies, ELISA Kits and Proteins.
Loading Loading
Order hotline:
phone +1 404 474 4654
fax +1 888 205 9894 (TF)
Details for Product No. ABIN500559

PUMA (N-Term) antibody

Request
Want additional data for this product?

The Independent Validation Initiative strives to provide you with high quality data. Find out more

Antigen
Epitope
»Alternatives N-Term
Reactivity
»Alternatives Human
Host
»Alternatives Rabbit
Clonality Polyclonal
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives Immunocytochemistry (ICC), Immunofluorescence (IF), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Western Blotting (WB)
Pubmed 3 references available
Catalog no. ABIN500559
Quantity 0.1 mg
Price
390.50 $   Plus shipping costs $45.00
Shipping to
Availability Will be delivered in 6 to 8 Business Days
Immunogen Synthetic peptide corresponding to 14 amino acids near the amino terminus of Human PUMA-alpha. .Remarks: This sequence is identical between alpha and beta forms of the PUMA proteins.
Isotype IgG
Specificity This antibody detects PUMA at N-term. Species Reactivity: Tested: Human.
Purification Peptide Affinity Chromatography
Background Apoptosis is related to many diseases and development. The p53 tumor-suppressor protein induces apoptosis through transcriptional activation of several genes. A novel p53 inducible pro-apoptotic gene was identified recently and designated PUMA (for p53 upregulated modulator of apoptosis) and bbc3 (for Bcl-2 binding component 3) in human and mouse (1-3). PUMA/bbc3 is one of the pro-apoptotic Bcl-2 family members including Bax and Noxa, which are also transcriptional targets of p53. The PUMA gene encodes two BH3 domain-containing proteins termed PUMA-alpha and PUMA-beta (1). PUMA proteins bind Bcl-2, localize to the mitochondria, and induce cytochrome c release and apoptosis in response to p53. PUMA may be a direct mediator of p53-induced apoptosis.
Alternate names: BBC3, Bcl-2-binding component 3, JFY-1, p53 up-regulated modulator of apoptosis
Gene ID 27113
NCBI Accession NP_055232
UniProt Q9BXH1
Research Area Cancer, Apoptosis/Necrosis, Metabolism
Application Notes ELISA. Western blot: 2 to 4 μg/ml, K562 cell lysate can be used as a positive control and a band atapproximately 23 kDa can be detected. Immunohistochemistry on paraffin sections. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Buffer PBS, 0.02% Sodium azide
Preservative Sodium azide
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer.
Expiry Date 12 months
Background publications Yu, Zhang, Hwang et al.: "PUMA induces the rapid apoptosis of colorectal cancer cells." in: Molecular cell, Vol. 7, Issue 3, pp. 673-82, 2001 (PubMed).

Nakano, Vousden: "PUMA, a novel proapoptotic gene, is induced by p53." in: Molecular cell, Vol. 7, Issue 3, pp. 683-94, 2001 (PubMed).

Han, Flemington, Houghton et al.: "Expression of bbc3, a pro-apoptotic BH3-only gene, is regulated by diverse cell death and survival signals." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 98, Issue 20, pp. 11318-23, 2001 (PubMed).

Alternatives for antigen "PUMA", type "Antibodies"
Hosts (52), (32), (1)
Reactivities (84), (38), (37), (1), (1)
Applications (81), (43), (12), (12), (11), (10), (3), (2), (1), (1)
Conjugates (1), (1), (1), (1), (1), (1), (1)
Epitopes (28), (12), (10), (7), (2), (1), (1), (1)
Beacon