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Details for Product No. ABIN500575

RAPTOR (Center) antibody

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Antigen
Epitope
»Alternatives Center
Reactivity
»Alternatives Human, Mouse (Murine)
Host
»Alternatives Rabbit
Clonality Polyclonal
Conjugate
»Alternatives Un-conjugated
Application
»Alternatives ELISA, Immunocytochemistry (ICC), Immunofluorescence (IF), Western Blotting (WB)
Pubmed 4 references available
Catalog no. ABIN500575
Quantity 0.1 mg
Price
390.50 $   Plus shipping costs $45.00
Shipping to
Availability Will be delivered in 6 to 8 Business Days
Immunogen Raptor antibody was raised against a 15 amino acid peptide from the central region of human Raptor.
Isotype IgG
Specificity This antibody detects RAPTOR at center. Raptor has multiple isoforms that may also be recognized by the antibody. Species Reactivity: Tested: Human, mouse
Purification Peptide affinity chromatography
Background The mammalian Target of Rapamycin (TOR, also known as mTOR) is an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors (reviewed in 1). Rapamycin inhibits TOR activity resulting in reduced cell growth and reduced rates of cell cycle and cell proliferation (reviewed in 2). Raptor (regulatory associated protein of TOR) is a TOR-binding protein essential for TOR signaling in vivo. It acts as a TOR scaffold protein whose binding by TOR substrates is necessary for effective TOR-catalyzed phosphorylation (3). These substrates include the ribosomal protein S6 kinase (RP S6K) and the eukaryotic initiation factor 4E binding protein 4EBP1, proteins necessary for cell growth and proliferation and responsive to nutrient and mitogen levels (4). Raptor binds these proteins through a common 5 amino acid TOR-signaling (TOS) motif, mutation of this motif prevents the TOR-dependent phosphorylation of these proteins (5).
Alternate names: KIAA1303, P150 target of rapamycin TOR-scaffold protein, RPTOR, Regulatory-associated protein of mTOR
Gene ID 57521
NCBI Accession Q8N122
UniProt Q8N122
Application Notes ELISA. Western blot: 2 μg/ml. Immunoflourescence. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Restrictions For Research Use only
Format Liquid
Buffer PBS containing 0.02% sodium azide
Preservative Sodium azide
Handling Advice Avoid repeated freezing and thawing.
Storage 4 °C/-20 °C
Storage Comment Store at 2 - 8 °C for up to one month or (in aliquots) at -20 °C for longer.
Expiry Date 12 months
Background publications Hara, Yonezawa, Weng et al.: "Amino acid sufficiency and mTOR regulate p70 S6 kinase and eIF-4E BP1 through a common effector mechanism." in: The Journal of biological chemistry, Vol. 273, Issue 23, pp. 14484-94, 1998 (PubMed).

Shamji, Nghiem, Schreiber: "Integration of growth factor and nutrient signaling: implications for cancer biology." in: Molecular cell, Vol. 12, Issue 2, pp. 271-80, 2003 (PubMed).

Yonezawa, Tokunaga, Oshiro et al.: "Raptor, a binding partner of target of rapamycin." in: Biochemical and biophysical research communications, Vol. 313, Issue 2, pp. 437-41, 2003 (PubMed).

Fingar, Blenis: "Target of rapamycin (TOR): an integrator of nutrient and growth factor signals and coordinator of cell growth and cell cycle progression." in: Oncogene, Vol. 23, Issue 18, pp. 3151-71, 2004 (PubMed).

Alternatives for antigen "RAPTOR", type "Antibodies"
Hosts (79), (3)
Reactivities (67), (54), (29), (25), (25), (24), (13), (12), (2), (1), (1), (1)
Applications (56), (27), (23), (20), (14), (10), (8), (4), (2), (2), (2), (1), (1)
Conjugates (2), (2), (2), (2), (2), (2), (2), (2), (2), (2), (2)
Epitopes (12), (10), (9), (7), (5), (2), (1), (1), (1), (1), (1), (1)
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